An updated linkage and comparative map of porcine chromosome 18

被引:16
作者
Campbell, EMG [1 ]
Fahrenkrug, SC [1 ]
Vallet, JL [1 ]
Smith, TPL [1 ]
Rohrer, GA [1 ]
机构
[1] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA
关键词
mapping; microsatellite; porcine;
D O I
10.1046/j.1365-2052.2001.00782.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Swine chromosome 18 (SSC18) has the poorest marker density in the USDA-MARC porcine linkage map. In order to increase the marker density, seven genes from human chromosome 7 (HSA7) expected to map to SSC18 were selected for marker development. The genes selected were: growth hormone releasing hormone receptor (GHRHR), GLI-Kruppel family member (GLI3), leptin (LEP), capping protein muscle Z-line alpha 2 subunit (CAPZA2), beta A inhibin (INHBA), T-cell receptor beta (TCRB) and T-cell receptor gamma (TCRG). Large-insert clones (YACs, BACs and cosmids) that contained these genes, as well as two previously mapped microsatellite markers (SW1808 and SW1984), were identified and screened for microsatellites. New microsatellite markers were developed from these clones and mapped. Selected clones were also physically assigned by fluorescence in situ hybridization (FISH). Fifteen new microsatellite markers were added to the SSC18 linkage map resulting in a map of 28 markers. Six genes have been included into the genetic map improving the resolution of the SSC18 and HSA7 comparative map. Assignment of TCRG to SSC9 has identified a break in conserved synteny between SSC18 and HSA7.
引用
收藏
页码:375 / 379
页数:5
相关论文
共 16 条
  • [1] Construction and characterization of a large insert porcine YAC library
    Alexander, LJ
    Smith, TPL
    Beattie, CW
    Broom, MF
    [J]. MAMMALIAN GENOME, 1997, 8 (01) : 50 - 51
  • [2] Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites
    Alexander, LJ
    Troyer, DL
    Rohrer, GA
    Smith, TPL
    Schook, LB
    Beattie, CW
    [J]. MAMMALIAN GENOME, 1996, 7 (05) : 368 - 372
  • [3] 2 HOMOLOGOUS GENES, ORIGINATED BY DUPLICATION, ENCODE THE HUMAN HNRNP PROTEIN-A2 AND PROTEIN-A1
    BIAMONTI, G
    RUGGIU, M
    SACCONE, S
    DELLAVALLE, G
    RIVA, S
    [J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (11) : 1996 - 2002
  • [4] Cepica S, 1999, ANIM GENET, V30, P476
  • [5] A porcine BAC library with tenfold genome coverage: a resource for physical and genetic map integration
    Fahrenkrug, SC
    Rohrer, GA
    Freking, BA
    Smith, TPL
    Osoegawa, K
    Shu, CL
    Catanese, JJ
    de Jong, PJ
    [J]. MAMMALIAN GENOME, 2001, 12 (06) : 472 - 474
  • [6] Expansion of the pig comparative map by expressed sequence tags (EST) mapping
    Fridolfsson, AK
    Hori, T
    Wintero, AK
    Fredholm, M
    Yerle, M
    Robic, A
    Andersson, L
    Ellegren, H
    [J]. MAMMALIAN GENOME, 1997, 8 (12) : 907 - 912
  • [7] Human and porcine correspondence of chromosome segments using bidirectional chromosome painting
    Goureau, A
    Yerle, M
    Schmitz, A
    Riquet, J
    Milan, D
    Pinton, P
    Frelat, G
    Gellin, J
    [J]. GENOMICS, 1996, 36 (02) : 252 - 262
  • [8] Green P., 1990, DOCUMENTATION CRI MA
  • [9] Chromosomal localization of homeobox genes and associated markers on porcine Chromosomes 3, 5, 12, 15, 16 and 18: Comparative mapping study with human and mouse
    LahbibMansais, Y
    Yerle, M
    Pinton, P
    Gellin, J
    [J]. MAMMALIAN GENOME, 1996, 7 (03) : 174 - 179
  • [10] A linkage map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs
    Mikawa, S
    Akita, T
    Hisamatsu, N
    Inage, Y
    Ito, Y
    Kobayashi, E
    Kusumoto, H
    Matsumoto, T
    Mikami, H
    Minezawa, M
    Miyake, M
    Shimanuki, S
    Sugiyama, C
    Uchida, Y
    Wada, Y
    Yanai, S
    Yasue, H
    [J]. ANIMAL GENETICS, 1999, 30 (06) : 407 - 417