Improved method for the removal of endotoxin from DNA

被引：27

作者：

Montbriand, PM ^{[1
]}

Malone, RW ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT MED PATHOL,GENE THERAPY PROGRAM,DAVIS,CA 95616

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 44卷 / 1-3期

关键词：

polymyxin B; endotoxin; plasmid purification; gene therapy;

D O I：

10.1016/0168-1656(95)00091-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Contaminating endotoxin in solutions used in gene therapy and genetic immunization can result in various deleterious effects both in vitro and in vivo. In order to avoid such complications, attempts were made to characterize the extent of the problem of endotoxin contamination and develop a solution to this problem. After screening for endotoxin in plasmid DNA preparations using the Limulus Amoebocyte Lysate (LAL) assay, nearly half of all samples displayed high endotoxin levels. Therefore, a simple one-step procedure was developed for the removal of endotoxin using a polymyxin B resin.

引用

页码：43 / 46

页数：4

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