Optimization for quantitative determination of four flavonoids in Epimedium by capillary zone electrophoresis coupled with diode array detection using central composite design

被引:115
作者
Liu, JJ [1 ]
Li, SP [1 ]
Wang, YT [1 ]
机构
[1] Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
关键词
capillary zone electrophoresis; quantitative determination; central composite design; flavonoids; Epimedium;
D O I
10.1016/j.chroma.2005.11.036
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Herba Epimedii (family Berberidaceae), Ying-Yang-Huo in Chinese, is a famous Chinese herbal medicine. Flavonoids are thought to be the major active components in it. A capillary zone electrophoresis (CZE) separation were developed for simultaneous determination of four flavonoids including icariin, epimedin A, epimedin B and epimedin C in Epimedium. The effects of the experimental variables on CZE had been optimized by using central composite design (CCD). The best separation of four flavonoids could be obtained using 50 mM borate buffer (pH 10.0) containing 22% acetontrile as modifier, while separation voltage was 15 kV and temperature was at 25 degrees C. The method developed is accurate, simple and reproducible, which could be used for quality control of Epimedium and its medical preparations. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:344 / 349
页数:6
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