Cochlin expression in vestibular endorgans obtained from patients with Meniere's disease

被引：32

作者：

Calzada, Audrey P. ^{[2
]}

Lopez, Ivan A. ^{[2
]}

Parrazal, Luis Beltran ^{[3
]}

Ishiyama, Akira ^{[2
]}

Ishiyama, Gail ^{[1
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Neurol, Reed Neurol Res Ctr, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Head & Neck Surg, Los Angeles, CA 90095 USA

[3] Univ Veracruzana, Ctr Cerebral Invest, Veracruz, Veracruz, Mexico

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurol, Los Angeles, CA 90095 USA

来源：

CELL AND TISSUE RESEARCH | 2012年 / 350卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Cochlin; Basement membrane; Meniere's disease; Inner ear; Vestibular endorgans; Human; INNER-EAR; BASEMENT-MEMBRANE; MESSENGER-RNA; EXTRACELLULAR-MATRIX; STRIA VASCULARIS; II COLLAGEN; MOUSE MODEL; GENE; LOCALIZATION; DEAFNESS;

D O I：

10.1007/s00441-012-1481-x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The distribution of cochlin and its associated basement membrane proteins (collagen IV, collagen II, laminin-beta 2, and nidogen-1) were evaluated in the vestibular endorgans of subjects with Meniere's disease and compared with normal specimens. Cochlin mRNA expression in vestibular endorgans from Meniere's disease specimens was also investigated. Specimens were obtained from patients who had Meniere's disease and who were undergoing ablative labyrinthectomy. Control specimens were obtained both from autopsy specimens with documented normal audiovestibular function and from patients undergoing labyrinthectomy for acoustic neuroma excision. In the normal control specimens, cochlin immunoreactivity was found evenly distributed in the stroma of the cristae ampullaris and maculae of the utricle. In Meniere's specimens, cochlin immunoreactivity was markedly increased; this was associated with an increase in cochlin mRNA expression as shown by real-time reverse transcription with the polymerase chain reaction. Collagen IV and laminin-beta 2 immunoreactivity was significantly decreased in Meniere's specimens. Nidogen-1 and collagen II immunoreactivity was unchanged in Meniere's specimens when compared with normal samples. Cochlin upregulation has been implicated in the hereditary audiovestibulopathy, DFNA9. The increased expression of cochlin and decreased expression of collagen IV and laminin in Meniere's disease are suggestive that the overexpression of cochlin contributes to the dysfunctional inner ear homeostasis seen in this disease.

引用

页码：373 / 384

页数：12

共 38 条

[1]

Baloh RW, 2001, ARCH NEUROL-CHICAGO, V258, P151

[2]

Cochlin deposits in the trabecular meshwork of the glaucomatous DBA/2J mouse [J].

Bhattacharya, SK ;

Annangudi, SP ;

Salomon, RG ;

Kuchtey, RW ;

Peachey, NS ;

Crabb, JW .

EXPERIMENTAL EYE RESEARCH, 2005, 80 (05) :741-744

[3]

Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork [J].

Bhattacharya, SK ;

Rockwood, EJ ;

Smith, SD ;

Bonilha, VL ;

Crabb, JS ;

Kuchtey, RW ;

Robertson, NG ;

Peachey, NS ;

Morton, CC ;

Crabb, JW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (07) :6080-6084

[4]

Proteomics, bioinformatics and targeted gene expression analysis reveals up-regulation of cochlin and identifies other potential biomarkers in the mouse model for deafness in usher syndrome type 1F [J].

Chance, Mark R. ;

Chang, Jinsook ;

Liu, Shuqing ;

Gokulrangan, Giridharan ;

Chen, Daniel H. -C. ;

Lindsay, Aaron ;

Geng, Ruishuang ;

Zheng, Qing Y. ;

Alagramam, Kumar .

HUMAN MOLECULAR GENETICS, 2010, 19 (08) :1515-1527

[5]

Medical Management Of Meniere's Disease [J].

Coelho, Daniel H. ;

Lalwani, Anil K. .

LARYNGOSCOPE, 2008, 118 (06) :1099-1108

[6]

COLOMBATTI A, 1991, BLOOD, V77, P2305

[7]

Ultrastructural, physiological, and molecular defects in the inner ear of a gene-knockout mouse model for autosomal Alport syndrome [J].

Cosgrove, D ;

Samuelson, G ;

Meehan, DT ;

Miller, C ;

McGee, J ;

Walsh, EJ ;

Siegel, M .

HEARING RESEARCH, 1998, 121 (1-2) :84-98

[8]

ECM homeostasis in renal diseases: a genomic approach [J].

Eikmans, M ;

Baelde, JJ ;

de Heer, E ;

Bruijn, JA .

JOURNAL OF PATHOLOGY, 2003, 200 (04) :526-536

[9]

High prevalence of symptoms of Meniere's disease in three families with a mutation in the COCH gene [J].

Fransen, E ;

Verstreken, M ;

Verhagen, WIM ;

Wuyts, FL ;

Huygen, PLM ;

D'Haese, P ;

Robertson, NG ;

Morton, CC ;

McGuirt, WT ;

Smith, RJH ;

Declau, F ;

Van de Heyning, PH ;

Van Camp, G .

HUMAN MOLECULAR GENETICS, 1999, 8 (08) :1425-1429

[10]

Extracellular matrices associated with the apical surfaces of sensory epithelia in the inner ear: Molecular and structural diversity [J].

Goodyear, RJ ;

Richardson, GP .

JOURNAL OF NEUROBIOLOGY, 2002, 53 (02) :212-227

← 1 2 3 4 →