Nanodiamond bioconjugate probes and their collection by electrophoresis

被引：87

作者：

Hens, Suzanne Ciftan ^{[1
]}

Cunningham, Garry ^{[1
]}

Tyler, Talmage ^{[1
]}

Moseenkov, Sergey ^{[2
]}

Kuznetsov, Vladimir ^{[2
]}

Shenderova, Olga ^{[1
]}

机构：

[1] Int Technol Ctr, Raleigh, NC 27617 USA

[2] Boreskov Inst Catalysis SB RAS, Novosibirsk 630090, Russia

来源：

关键词：

Diamond; Nanotechnology; Surface Chemistry; Bioconjugation; Electrophoresis; Streptavidin;

D O I：

10.1016/j.diamond.2008.03.020

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The application of detonation nanodiamonds (NDs) as probes for protein capture and electrophoretic collection was investigated. NDs were chemically modified in a series of reactions to produce a ND-NH2 product that had increased chemical homogeneity. The product was characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). FTIR spectra were taken using an IR vacuum cuvette and the samples were dehydrated at different temperatures. The ND-NH2 product was capable of conjugating to N-hydroxysuccinimide derivatives of TAMRA and biotin. We calculated that the number of chemically attached TAMRA molecules on ND-NH2 was similar to 1 molecule/nm(2). The singly conjugated TAMRA-ND (T-ND) and doubly conjugated TAMRA-ND-Biotin (T-ND-B) products formed stable aqueous colloidal suspensions. T-ND and T-ND-B were collected on planar electrodes and silicon field tip arrays using a field of 10 V/cm. The rate of collection for the aminated ND is dependent upon field strength and an exponential decrease in current was observed as a function of time. Streptavidin was captured by the T-ND-B bioconjugate probe and this nanoparticle-protein complex was collected from solution by electrophoresis. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1858 / 1866

页数：9

共 35 条

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