HPLC-Fluorescence Method for Measurement of the Uremic Toxin Indoxyl Sulfate in Plasma

被引：47

作者：

Al Za'abi, Mohammed ^{[1
]}

Ali, Badreldin ^{[1
]}

Al Toubi, Mohammed ^{[2
]}

机构：

[1] Sultan Qaboos Univ, Dept Pharmacol & Clin Pharm, Coll Med & Hlth Sci, Muscat, Oman

[2] Sultan Qaboos Univ, Dept Biochem, Coll Med & Hlth Sci, Muscat, Oman

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2013年 / 51卷 / 01期

关键词：

LIQUID-CHROMATOGRAPHY; SERUM; BINDING; INDOLE;

D O I：

10.1093/chromsci/bms103

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and reproducible high-performance liquid chromatography fluorescence method was developed and validated for quantitative determination of indoxyl sulfate in plasma using methyl paraben as an internal standard. The separation was carried out on an OSD-2 C18 Spherisorb column by isocratic elusion with sodium acetate buffer (pH 4.5) and acetonitrile (10:90, v/v) as the mobile phase. The method was validated and found to be linear in the range of 2.5 to 50 mu M. The limit of quantification was 2.0 mu M. The variations for intra-day and inter-day precision were less than 10.1%, and the accuracy values were between 93.4 and 102.5%. The extraction recoveries were more than 89%.

引用

页码：40 / 43

页数：4

共 11 条

[1]

Anavekar NS, 2004, NEW ENGL J MED, V351, P1285, DOI 10.1056/NEJMoa041365

[2] Sulfation of indoxyl by human and rat aryl (phenol) sulfotransferases to form indoxyl sulfate [J].