New Tools for Quantifying HIV-1 Reservoirs: Plasma RNA Single Copy Assays and Beyond

被引：21

作者：

Hilldorfer, Benedict B. ^{[1
]}

Cillo, Anthony R. ^{[1
]}

Besson, Guillaume J. ^{[1
]}

Bedison, Margaret Anne ^{[1
]}

Mellors, John W. ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Med, Div Infect Dis, Pittsburgh, PA 15261 USA

来源：

CURRENT HIV/AIDS REPORTS | 2012年 / 9卷 / 01期

关键词：

Single copy assay; Residual viremia; HIV reservoirs; HIV quantification; Ultrasensitive PCR; HIV treatment intensification;

D O I：

10.1007/s11904-011-0104-6

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Quantification of plasma HIV-1 RNA below the limit of FDA-approved assays by a single copy quantitative PCR assays (SCA) has provided significant insights into HIV-1 persistence despite potent antiretroviral therapy as well as a means to assess the impact of therapeutic strategies, such as treatment intensification, on residual viremia. In this review, we discuss insights gained from plasma HIV-1 RNA SCA and highlight the need for additional assays to characterize better the cellular and tissue reservoirs of HIV-1. Accurate, reproducible, and sensitive assays to quantify HIV-1 reservoirs, before and after therapeutic interventions, are essential tools in the quest for a cure of HIV-1 infection.

引用

页码：91 / 100

页数：10

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