A high-throughput approach for measuring temporal changes in the interactome

被引：268

作者：

Kristensen, Anders R. ^{[1
,2
]}

Gsponer, Joerg ^{[1
,2
]}

Foster, Leonard J. ^{[1
,2
]}

机构：

[1] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada

[2] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V5Z 1M9, Canada

来源：

NATURE METHODS | 2012年 / 9卷 / 09期

基金：

加拿大创新基金会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; IN-VIVO; COMPLEXES;

D O I：

10.1038/nmeth.2131

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Interactomes are often measured using affinity purification-mass spectrometry (AP-MSMS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MSMS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.

引用

页码：907 / +

页数：5

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