The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner

被引:24
作者
Brunel, F
Zakin, MM
Buc, H
Buckle, M
机构
[1] INST PASTEUR,UNITE EXPRESS GENES EUCARYOTES,F-75015 PARIS,FRANCE
[2] INST PASTEUR,UNITE PHYSICOCHIM MACROMOLEC BIOL,URA 1149,F-75015 PARIS,FRANCE
关键词
D O I
10.1093/nar/24.9.1608
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV cross-linking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.
引用
收藏
页码:1608 / 1615
页数:8
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