Activation of protease by sol-gel entrapment into organically modified hybrid silicates

被引：11

作者：

Furukawa, S ^{[1
]}

Ono, T ^{[1
]}

Ijima, H ^{[1
]}

Kawakami, K ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Mat Proc Engn, Higashi Ku, Fukuoka 8128581, Japan

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 01期

关键词：

organic-inorganic hybrid silicate; organic solvent; protease; sol-gel method; transesterification;

D O I：

10.1023/A:1013876313806

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methyl-1-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85 degreesC.

引用

页码：13 / 16

页数：4

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