Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima

被引:95
作者
Huang, Shyh-Shyun [1 ]
Chiu, Chuan-Sung [2 ]
Lin, Tsung-Hui [3 ]
Lee, Min-Min [4 ]
Lee, Chao-Ying [1 ]
Chang, Shu-Jen [1 ]
Hou, Wen-Chi [5 ]
Huang, Guan-Jhong [6 ]
Deng, Jeng-Shyan [4 ]
机构
[1] China Med Univ, Sch Pharm, Coll Pharm, Taichung 404, Taiwan
[2] Hsin Sheng Coll Med Care & Management, Dept Nursing, Tao Yuan 325, Taiwan
[3] MingDao Univ, Dept Leisure Recreat & Holist Wellness, Changhua 523, Taiwan
[4] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan
[5] Taipei Med Univ, Grad Inst Pharmacognosy, Taipei, Taiwan
[6] China Med Univ, Sch Chinese Pharmaceut Sci & Chinese Med Resource, Coll Pharm, Taichung 404, Taiwan
关键词
Chinese medicine; Centipeda minima; Antioxidant activity; Anti-inflammatory activity; INDUCED PAW EDEMA; IN-VITRO; SESQUITERPENE LACTONE; ANTI-INFLAMMATION; FREE-RADICALS; NITRIC-OXIDE; ACID; MECHANISMS; RATS;
D O I
10.1016/j.jep.2013.03.025
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
Ethnopharmacological relevance: Centipeda minima (L) is traditionally used in Chinese folk medicine for the treatments of rhinitis, sinusitis, relieving pain, reducing swelling, and treating cancer for a long history in Taiwan. However, there is no scientific evidence which supports the use in the literature. Aim of the study: The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of the aqueous extract of Centipeda minima (ACM). Materials and methods: The following activities were investigated: antioxidant activities 12,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), DPPH (1,1-dipheny1-2-picrylhydrazyl)], and anti-inflammatory [lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 macrophages and paw-edema induced by lambda-carrageenan (Carr)]. We also investigated the anti-inflammatory mechanism of ACM via studies of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA) in the edema paw. Serum NO, tumor necrosis factor alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) were also measured in vivo. In HPLC analysis, the fingerprint chromatogram of ACM was established. Results: ACM showed the highest TEAC and DPPH radical scavenging activities, respectively. ACM also had highest contents of polyphenol and flavonoid contents. We evaluated that ACM and the reference compound of protocatechualdehyde and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Administration of ACM showed a concentration dependent inhibition on paw edema development after Carr treatment in mice. The anti-inflammatory effects of ACM could be via NO, TNF-alpha, and IL-1 beta suppressions and associated with the increase in the activities of antioxidant enzymes. Western blotting revealed that ACM decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Conclusions: Anti-inflammatory mechanisms of ACM might be correlated to the decrease in the level of Malondialdehyde (MDA), iNOS, and COX-2 via increasing the activities of CAT, SOD, and GPx in the edema paw. Overall, the results showed that ACM demonstrated antioxidant and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:395 / 405
页数:11
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