Planar waveguides as high performance sensing platforms for fluorescence-based multiplexed oligonucleotide hybridization assays

被引:66
作者
Budach, W [1 ]
Abel, AP [1 ]
Bruno, AE [1 ]
Neuschäfer, D [1 ]
机构
[1] Novartis Pharma AG, DMPK, CH-4002 Basel, Switzerland
关键词
D O I
10.1021/ac990092e
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
16-Mer and 22-mer oligonucleotide capture probes with an amino function at the 5'-end were covalently immobilized on (3-glycidoxypropyl)trimethoxysilane (GOPTS) silanized planar waveguides in "checkerboard"-style 2-dimensional arrays by means of ink-jet printing in order to demonstrate the potential of multiplexed planar waveguide biosensing. The laser-induced fluorescence was collected by a CCD camera, providing quantitative information regarding the intensity of individual sensing pads. In model hybridization assays with Cy5-labeled complementary oligonucleotides, sample concentrations down to 50 fM were successfully detected within 12 min. The overall spot-to-spot variability of the ink-jet immobilization in combination with amino functionalized oligonucleotides and GOPTS silanized waveguides was found to be about 12% and almost independent of the formation of an equilibrium between the labeled analyte and capture probes. Both pipet-made and ink-jet-made oligonucleotide spots showed comparable assay performances.
引用
收藏
页码:3347 / 3355
页数:9
相关论文
共 31 条
  • [1] Fiber-optic evanescent wave biosensor for the detection of oligonucleotides
    Abel, AP
    Weller, MG
    Duveneck, GL
    Ehrat, M
    Widmer, HM
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (17) : 2905 - 2912
  • [2] APEL O, UNPUB APPL OPT
  • [3] BALDESCHWIELER JD, 1995, Patent No. 25116
  • [4] Surface plasmon resonance multisensing
    Berger, CEH
    Beumer, TAM
    Kooyman, RPH
    Greve, J
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (04) : 703 - 706
  • [5] Printing patterns of proteins
    Bernard, A
    Delamarche, E
    Schmid, H
    Michel, B
    Bosshard, HR
    Biebuyck, H
    [J]. LANGMUIR, 1998, 14 (09) : 2225 - 2229
  • [6] BRUNO AE, 1998, MICRO TOTAL ANAL SYS, P281
  • [7] Chiu DT, 1997, CHEM-EUR J, V3, P335
  • [8] Dammann H., 1971, Optics Communications, V3, P312, DOI 10.1016/0030-4018(71)90095-2
  • [9] Capillary flow as the cause of ring stains from dried liquid drops
    Deegan, RD
    Bakajin, O
    Dupont, TF
    Huber, G
    Nagel, SR
    Witten, TA
    [J]. NATURE, 1997, 389 (6653) : 827 - 829
  • [10] Duebendorfer J, 1997, J Biomed Opt, V2, P391, DOI 10.1117/12.281487