Constructing immortalized human cell lines

被引:40
作者
Yeager, TR [1 ]
Reddel, RR [1 ]
机构
[1] Childrens Med Res Inst, Sydney, NSW 2145, Australia
基金
英国医学研究理事会;
关键词
D O I
10.1016/S0958-1669(99)00011-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Obtaining an abundant supply of human cells with specific differentiated properties is a key issue in many research studies and biotechnology applications. Recent advances in this area include the finding that forced expression of the enzyme telomerase can greatly increase the proliferative potential of at least some types of human cells, and also the development of methods for culturing human stem cells.
引用
收藏
页码:465 / 469
页数:5
相关论文
共 47 条
[1]  
[Anonymous], SCIENCE
[2]   IMMORTALIZATION OF NORMAL HUMAN FIBROBLASTS BY TREATMENT WITH 4-NITROQUINOLINE 1-OXIDE [J].
BAI, L ;
MIHARA, K ;
KONDO, Y ;
HONMA, M ;
NAMBA, M .
INTERNATIONAL JOURNAL OF CANCER, 1993, 53 (03) :451-456
[3]  
BISCHOFF FZ, 1990, CANCER RES, V50, P7979
[4]   Extension of life-span by introduction of telomerase into normal human cells [J].
Bodnar, AG ;
Ouellette, M ;
Frolkis, M ;
Holt, SE ;
Chiu, CP ;
Morin, GB ;
Harley, CB ;
Shay, JW ;
Lichtsteiner, S ;
Wright, WE .
SCIENCE, 1998, 279 (5349) :349-352
[5]  
Boyer SN, 1996, CANCER RES, V56, P4620
[6]   Increased p16 expression with first senescence arrest in human mammary epithelial cells and extended growth capacity with p16 inactivation [J].
Brenner, AJ ;
Stampfer, MR ;
Aldaz, CM .
ONCOGENE, 1998, 17 (02) :199-205
[7]   SV40-INDUCED IMMORTALIZATION OF HUMAN-CELLS [J].
BRYAN, TM ;
REDDEL, RR .
CRITICAL REVIEWS IN ONCOGENESIS, 1994, 5 (04) :331-357
[8]   Telomere maintenance mechanisms and cellular immortalization [J].
Colgin, LM ;
Reddel, RR .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (01) :97-103
[9]   Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization [J].
Counter, CM ;
Hahn, WC ;
Wei, WY ;
Caddle, SD ;
Beijersbergen, RL ;
Lansdorp, PM ;
Sedivy, JM ;
Weinberg, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14723-14728
[10]  
De Silva R., 1995, Methods in Cell Science, V17, P75