Enhanced phytoextraction of an agricultural Cr- and Pb-contaminated soil by bioaugmentation with siderophore-producing bacteria

被引:224
作者
Braud, Armelle [1 ]
Jezequel, Karine [1 ]
Bazot, Stephane [1 ]
Lebeau, Thierry [1 ]
机构
[1] Univ Haute Alsace, EDBS, F-68008 Colmar, France
关键词
Pseudomonas; Ralstonia; Heavy metal; Metal mobility; Maize; Cell immobilization; PSEUDOMONAS-FLUORESCENS CELLS; HEAVY-METAL; ROOT COLONIZATION; PLANT-GROWTH; SURVIVAL; PHYTOREMEDIATION; BIOREMEDIATION; AERUGINOSA; RELEASE; MAIZE;
D O I
10.1016/j.chemosphere.2008.09.013
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Bioaugmentation-assisted phytoextraction may enhance the phytoextraction efficiency thanks to larger metal mobilization by microbial metabolites. Green fluorescent protein-tagged cells of Pseudomonas aeruginosa, Pseudo m on as fluorescens or Ralstonia metallidurans, able to produce siderophores, were inoculated in an agricultural soil containing Cr (488 mg kg(-1)) and Pb (382 mg kg(-1)) and maize was cultivated. Bacteria were inoculated as free or immobilized cells in Ca-alginate beads, with skim milk in the aim at improving both the bacterial survival and the in situ siderophore production. Skim milk addition increased inoculated Pseudomonads concentration in soil. Soil inoculation with free cells of R. metallidurans supplied with skim milk increased Cr accumulation in maize shoots by a factor of 5.2 and inoculation with immobilized P. aeruginosa cells supplied with skim milk increased Cr and Pb uptake by maize shoots by a factor of 5.4 and 3.8, respectively. However total metal taken up by the whole plant decreases almost always with bioaugmentation. Translocation factor also increased with P. aeruginosa or R. metallidurans by a factor of 6 up to 7. Inoculated bacteria concentration in soil was correlated with metals in the exchangeable fraction. Cr and Pb concentrations in the exchangeable fraction were correlated with metal contents in shoots or roots. Our results suggest that bioaugmentation-assisted phytoextraction is a relevant method in the aim at increasing the phytoextraction rate which usually limits the use of phytoremediation technologies. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:280 / 286
页数:7
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