The influence of architecture on degradation and tissue ingrowth into three-dimensional poly(lactic-co-glycolic acid) scaffolds in vitro and in vivo

被引:110
作者
Cao, Y
Mitchell, G
Messina, A
Price, L
Thompson, E
Penington, A
Morrison, W
O'Connor, A
Stevens, G
Cooper-White, J
机构
[1] Univ Melbourne, Dept Chem & Biomol Engn, Parkville, Vic 3010, Australia
[2] Bernard OBrien Inst Microsurg, Fitzroy, Vic 3065, Australia
[3] Univ Melbourne, St Vincents Hosp, Dept Surg, Fitzroy, Vic 3065, Australia
[4] Univ Bath, Dept Chem Engn, Bath BA2 7AY, Avon, England
基金
澳大利亚研究理事会;
关键词
polyglycolic acid and polylactic acid scaffold; in vitro and in vivo tests; biodegradation; mechanical properties; foreign body response;
D O I
10.1016/j.biomaterials.2005.12.015
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The in vitro and in vivo degradation properties of poly(lactic-co-glycolic acid) (PLGA) scaffolds produced by two different technologies-therm ally induced phase separation (TIPS), and solvent casting and particulate leaching (SCPL) were compared. Over 6 weeks, in vitro degradation produced changes in SCPL scaffold dimension, mass, internal architecture and mechanical properties. TIPS scaffolds produced far less changes in these parameters providing significant advantages over SCPL. In vivo results were based on a microsurgically created arteriovenous (AV) loop sandwiched between two TIPS scaffolds placed in a polycarbonate chamber under rat groin skin. Histologically, a predominant foreign body giant cell response and reduced vascularity was evident in tissue ingrowth between 2 and 8 weeks in TIPS scaffolds. Tissue death occurred at 8 weeks in the smallest pores. Morphometric comparison of TIPS and SCPL scaffolds indicated slightly better tissue ingrowth but greater loss of scaffold structure in SCPL scaffolds. Although advantageous in vitro, large surface area:volume ratios and varying pore sizes in PLGA TIPS scaffolds mean that effective in vivo (AV loop) utilization will only be achieved if the foreign body response can be significantly reduced so as to allow successful vascularisation, and hence sustained tissue growth, in pores less than 300 mu m. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2854 / 2864
页数:11
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