Disruption of cortical microtubules by overexpression of green fluorescent protein-tagged α-tubulin 6 causes a marked reduction in cell wall synthesis

被引：47

作者：

Burk, DH ^{[1
]}

Zhong, RQ ^{[1
]}

Morrison, WH ^{[1
]}

Ye, ZH ^{[1
]}

机构：

[1] USDA, Agr Res Serv, Richard B Russell Agr Res Ctr, Athens, GA 30604 USA

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2006年 / 48卷 / 01期

关键词：

Arabidopsis thaliana; cell elongation; cell wall synthesis; cortical microtubules; microtubule organization;

D O I：

10.1111/j.1744-7909.2006.00202.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been known that the transverse orientation of cortical microtubules (MTs) along the elongation axis is essential for normal cell morphogenesis, but whether cortical MTs are essential for normal cell wall synthesis is still not clear. In the present study, we have investigated whether cortical MTs affect cell wall synthesis by direct alteration of the cortical MT organization in Arabidopsis thaliana. Disruption of the cortical MT organization by expression of an excess amount of green fluorescent protein-tagged alpha-tubulin 6 (GFP-TUA6) in transgenic Arabidopsis plants was found to cause a marked reduction in cell wall thickness and a decrease in the cell wall sugars glucose and xylose. Concomitantly, the stem strength of the GFP-TUA6 overexpressors was markedly reduced compared with the wild type. In addition, expression of excess GFP-TUA6 results in an alteration in cell morphogenesis and a severe effect on plant growth and development. Together, these results suggest that the proper organization of cortical MTs is essential for the normal synthesis of plant cell walls.

引用

页码：85 / 98

页数：14

共 33 条

[1] Altered microtubule dynamics by expression of modified α-tubulin protein causes right-handed helical growth in transgenic Arabidopsis plants [J].

Abe, T ;

Hashimoto, T .

PLANT JOURNAL, 2005, 43 (02) :191-204

[2] Microtubule defects and cell morphogenesis in the lefty1lefty2 tubulin mutant of Arabidopsis thaliana [J].

Abe, T ;

Thitamadee, S ;

Hashimoto, T .

PLANT AND CELL PHYSIOLOGY, 2004, 45 (02) :211-220

[3] Disorganization of cortical microtubules stimulates tangential expansion and reduces the uniformity of cellulose microfibril alignment among cells in the root of Arabidopsis [J].

Baskin, TI ;

Beemster, GTS ;

Judy-March, JE ;

Marga, F .

PLANT PHYSIOLOGY, 2004, 135 (04) :2279-2290

[4] On the alignment of cellulose microfibrils by cortical microtubules: a review and a model [J].

Baskin, TI .

PROTOPLASMA, 2001, 215 (1-4) :150-171

[5]

BECHTOLD N, 1994, GENE TRANSFER PLANTS, V1, P19

[6] BOTERO1 is required for normal orientation of cortical microtubules and anisotropic cell expansion in Arabidopsis [J].

Bichet, A ;

Desnos, T ;

Turner, S ;

Grandjean, O ;

Höfte, H .

PLANT JOURNAL, 2001, 25 (02) :137-148

[7] The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth [J].

Bouquin, T ;

Mattsson, O ;

Næsted, H ;

Foster, R ;

Mundy, J .

JOURNAL OF CELL SCIENCE, 2003, 116 (05) :791-801

[8] Alteration of oriented deposition of cellulose microfibrils by mutation of a katanin-like microtubule-severing protein [J].

Burk, DH ;

Ye, ZH .

PLANT CELL, 2002, 14 (09) :2145-2160

[9] A katanin-like protein regulates normal cell wall biosynthesis and cell elongation [J].

Burk, DH ;

Liu, B ;

Zhong, RQ ;

Morrison, WH ;

Ye, ZH .

PLANT CELL, 2001, 13 (04) :807-827

[10] The cortical microtubule array: From dynamics to organization [J].

Dixit, R ;

Cyr, R .

PLANT CELL, 2004, 16 (10) :2546-2552

← 1 2 3 4 →