Antitumor activity of phenylahistin in vitro and in vivo

被引：79

作者：

Kanoh, K ^{[1
]}

Kohno, S ^{[1
]}

Katada, J ^{[1
]}

Hayashi, Y ^{[1
]}

Muramatsu, M ^{[1
]}

Uno, I ^{[1
]}

机构：

[1] Nippon Steel Corp, Adv Technol Res Labs, Life Sci Res Ctr, Nakahara Ku, Kawasaki, Kanagawa 2110035, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 06期

关键词：

phenylahistin; enantiomers; cell cycle inhibitor; antitumor activity;

D O I：

10.1271/bbb.63.1130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenylahistin is a new cell cycle inhibitor produced by Aspergillus ustus. Since phenylahistin was produced as a scalemic mixture of (-)-phenylahistin and its enantiomer, we separated each enantiomer and evaluated their antitumor activity in vitro. (-)-Phenylahistin exhibited antitumor activity against 8 tumor cell lines with IC50 values ranging from 1.8 x 10(-7) to 3.7 x 10(-6), while (+)-phenylahistin exhibited 33-100-fold less potent activity than (-)-phenylahistin did. (-)-Phenylahistin also showed antitumor activity against P388 leukemia and Lewis lung carcinoma cells in vivo.

引用

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页码：1130 / 1133

页数：4

相关论文

共 9 条

[1] TOXICITY OF ECHINULIN FROM ASPERGILLUS-CHEVALIERI IN RABBITS [J].

ALI, M ;

MOHAMMED, N ;

ALNAQEEB, MA ;

HASSAN, RAH ;

AHMAD, HSA .

TOXICOLOGY LETTERS, 1989, 48 (03) :235-241

[2] RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].

DENIZOT, F ;

LANG, R .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) :271-277

[3] SCREENING METHODS FOR THE DETECTION OF 13 COMMON MYCOTOXINS [J].

GORSTALLMAN, CP ;

STEYN, PS .

JOURNAL OF CHROMATOGRAPHY, 1979, 175 (02) :325-331

[4] (-)-Phenylahistin arrests cells in mitosis by inhibiting tubulin polymerization [J].

Kanoh, K ;

Kohno, S ;

Katada, J ;

Takahashi, J ;

Uno, I .

JOURNAL OF ANTIBIOTICS, 1999, 52 (02) :134-141

[5] (-)-phenylahistin: A new mammalian cell cycle inhibitor produced by Aspergillus ustus [J].

Kanoh, K ;

Kohno, S ;

Asari, T ;

Harada, T ;

Katada, J ;

Muramatsu, M ;

Kawashima, H ;

Sekiya, H ;

Uno, I .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1997, 7 (22) :2847-2852

[6] COMPUTER-AIDED ANALYSIS OF CELL-CYCLE PHASE FROM CYTOPHOTOMETRIC HISTOGRAM [J].

KOSUGI, Y ;

IKEBE, J ;

SEKINE, M ;

MUSHA, T ;

SHITARA, N ;

KOHNO, T ;

TAKAKURA, K .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 1978, 25 (05) :429-434

[7] Using direct electrospray mass spectrometry in taxonomy and secondary metabolite profiling of crude fungal extracts [J].

Smedsgaard, J ;

Frisvad, JC .

JOURNAL OF MICROBIOLOGICAL METHODS, 1996, 25 (01) :5-17

[8]

STEYN PS, 1971, TETRAHEDRON LETT, P3331, DOI 10.1016/S0040-4039(01)97170-9

[9] NEUROTOXICAL STUDIES ON FUMITREMORGIN-A, A TREMORGENIC MYCOTOXIN, ON MICE [J].

YAMAZAKI, M ;

SUZUKI, S ;

KUKITA, KI .

JOURNAL OF PHARMACOBIO-DYNAMICS, 1979, 2 (02) :119-125