Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus

被引:8
作者
Charron, C
Talfournier, F
Isupov, MN
Branlant, G
Littlechild, JA
Vitoux, B
Aubry, A
机构
[1] Univ Nancy 1, CNRS, UPRESA 7036, Lab Cristallog & Modelisat Mat Mineraux & Biol, F-54506 Vandoeuvre Nancy, France
[2] Univ Nancy 1, CNRS, UMR 7567, F-54506 Vandoeuvre Nancy, France
[3] Univ Exeter, Sch Chem, Exeter EX4 4QD, Devon, England
[4] Univ Exeter, Sch Biol Sci, Exeter EX4 4QD, Devon, England
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词
D O I
10.1107/S0907444999005363
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The homotetrameric holo-D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus has been crystallized in the presence of NADP(+) using the hanging-drop vapour-diffusion method. Crystals grew from a solution containing 2-methyl-2,4-pentanediol and magnesium acetate. A native data set has been collected to 2.1 Angstrom using synchrotron radiation and cryocooling. Diffraction data have been processed in the orthorhombic system (space group P2(1)2(1)2) with unit-cell dimensions a = 136.7, b = 153.3, c = 74.9 Angstrom and one tetramer per asymmetric unit.
引用
收藏
页码:1353 / 1355
页数:3
相关论文
共 30 条
[1]   NUCLEOTIDE-SEQUENCE AND MOLECULAR EVOLUTION OF THE GENE CODING FOR GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN THE THERMOACIDOPHILIC ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS [J].
ARCARI, P ;
DELLORUSSO, A ;
IANNICIELLO, G ;
GALLO, M ;
BOCCHINI, V .
BIOCHEMICAL GENETICS, 1993, 31 (5-6) :241-251
[2]   Comparison of the structures of wild-type and a N313T mutant of Escherichia coli glyceraldehyde 3-phosphate dehydrogenases: Implication for NAD binding and cooperativity [J].
Duee, E ;
OlivierDeyris, L ;
Fanchon, E ;
Corbier, C ;
Branlant, G ;
Dideberg, O .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 257 (04) :814-838
[3]   EXPRESSION OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE FROM THE EXTREMELY THERMOPHILIC ARCHAEBACTERIUM METHANOTHERMUS-FERVIDUS IN ESCHERICHIA-COLI - ENZYME-PURIFICATION, CRYSTALLIZATION, AND PRELIMINARY CRYSTAL DATA [J].
FABRY, S ;
LEHMACHER, A ;
BODE, W ;
HENSEL, R .
FEBS LETTERS, 1988, 237 (1-2) :213-217
[4]   PRIMARY STRUCTURE OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE DEDUCED FROM THE NUCLEOTIDE-SEQUENCE OF THE THERMOPHILIC ARCHAEBACTERIUM METHANOTHERMUS-FERVIDUS [J].
FABRY, S ;
HENSEL, R .
GENE, 1988, 64 (02) :189-197
[5]   PURIFICATION AND CHARACTERIZATION OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE THERMOPHILIC ARCHAEBACTERIUM METHANOTHERMUS-FERVIDUS [J].
FABRY, S ;
HENSEL, R .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 165 (01) :147-155
[6]   NUCLEOTIDE-SEQUENCE OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE FROM THE MESOPHILIC METHANOGENIC ARCHAEBACTERIA METHANOBACTERIUM-BRYANTII AND METHANOBACTERIUM-FORMICICUM - COMPARISON WITH THE RESPECTIVE GENE STRUCTURE OF THE CLOSELY RELATED EXTREME THERMOPHILE METHANOTHERMUS-FERVIDUS [J].
FABRY, S ;
LANG, J ;
NIERMANN, T ;
VINGRON, M ;
HENSEL, R .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 179 (02) :405-413
[7]   Characterization, crystallization and preliminary x-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus [J].
Fleming, TM ;
Jones, CE ;
Piper, PW ;
Cowan, DA ;
Isupov, MN ;
Littlechild, JA .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1998, 54 :671-674
[8]   MOLECULAR SYMMETRY OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM BACILLUS-COAGULANS [J].
GRIFFITH, JP ;
LEE, B ;
MURDOCK, AL ;
AMELUNXEN, RE .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 169 (04) :963-974
[9]  
HARRIS JI, 1976, ENZYMES, V13, P1
[10]   SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].
JANCARIK, J ;
KIM, SH .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411