Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

被引：18

作者：

Vianna, Julia Silveira ^{[1
]}

Ramis, Ivy Bastos ^{[2
]}

Bartolomeu Halicki, Priscila Cristina ^{[1
]}

Gastal, Otavio Leite ^{[3
]}

Silva, Renato Azevedo ^{[3
]}

Salomao Junior, Jose ^{[4
]}

dos Santos, Deise Machado ^{[4
]}

Chaves, Ana Lucia ^{[4
]}

Juliano, Carlos Renan ^{[4
]}

Jannke, Heitor Alberto ^{[3
]}

da Silva, Lande Vieira, Jr. ^{[2
]}

Von Groll, Andrea ^{[2
]}

Almeida da Silva, Pedro Eduardo ^{[2
]}

机构：

[1] Univ Fed Pelotas UFPel, Programa Pos Grad Biotecnol, Pelotas, RS, Brazil

[2] Fundacao Univ Fed Rio Grande, Nucleo Pesquisas Microbiol Med, Rio Grande, RS, Brazil

[3] Univ Catolica Pelotas, HUSFP, Pelotas, RS, Brazil

[4] Fundacao Univ Fed Rio Grande, Hosp Univ Dr Miguel Riet Correa Jr, Rio Grande, RS, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2015年 / 83卷 / 02期

关键词：

Helicobacter pylori; cagA protein; Amino acid repeat sequence; Gastritis; Peptic ulcer; PEPTIC-ULCER; CLINICAL-RELEVANCE; SYDNEY SYSTEM; GENE; REGION; PATHOGENICITY; ASSOCIATION; INFECTION; GENOTYPES; ANTIGEN;

D O I：

10.1016/j.diagmicrobio.2015.05.017

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

CagA of Helicobacter pylori undergoes tyrosine phosphorylation in a region containing differing numbers of repeat sequences (EPIYAs), which can result in a modulation of the inflammatory response. This study investigated whether the presence of CagA EPIYA variations in strains of H. pylori that are positive for this region contributes to differing degrees of disease severity in the gastric mucosa. In this study, 157 H. pylori-positive patients were included, and of those, 40.8% (64/157) were infected with cagA-positive strains, which were assayed for the presence of CagA EPIYA-ABC, EPIYA-ABCC, and EPIYA-ABCCC. Peptic ulcers were significantly more prevalent in patients infected with strains containing CagA EPIYA-ABCC/ABCCC than in those with CagA EPIYA ABC strains (P = 0.044). This suggests that the number of repetitions of EPIYA-C influences the development of gastroduodenal lesions, highlighting the importance and usefulness of evaluating the cagA gene sequence when making therapeutic intervention decisions in patients infected with H. pylori. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：89 / 92

页数：4

共 35 条

[1]

Abdullah Shahla M, 2012, Arab J Gastroenterol, V13, P166, DOI 10.1016/j.ajg.2012.12.001

[2]

Causal role of Helicobacter pylori infection in gastric cancer [J].

Ando, Takafumi ;

Goto, Yasuyuki ;

Maeda, Osamu ;

Watanabe, Osamu ;

Ishiguro, Kazuhiro ;

Goto, Hidemi .

WORLD JOURNAL OF GASTROENTEROLOGY, 2006, 12 (02) :181-186

[3]

MOSAICISM IN VACUOLATING CYTOTOXIN ALLELES OF HELICOBACTER-PYLORI - ASSOCIATION OF SPECIFIC VACA TYPES WITH CYTOTOXIN PRODUCTION AND PEPTIC-ULCERATION [J].

ATHERTON, JC ;

CAO, P ;

PEEK, RM ;

TUMMURU, MKR ;

BLASER, MJ ;

COVER, TL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :17771-17777

[4]

The Versatility of Helicobacter pylori CagA Effector Protein Functions: The Master Key Hypothesis [J].

Backert, Steffen ;

Tegtmeyer, Nicole ;

Selbach, Matthias .

HELICOBACTER, 2010, 15 (03) :163-176

[5]

Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms [J].

Basso, Daniela ;

Zambon, Carlo-Federico ;

Letley, Darren P. ;

Stranges, Alessia ;

Marchet, Alberto ;

Rhead, Joanne L. ;

Schiavon, Stefania ;

Guariso, Graziella ;

Ceroti, Marco ;

Nitti, Donato ;

Rugge, Massimo ;

Plebani, Mario ;

Atherton, John C. .

GASTROENTEROLOGY, 2008, 135 (01) :91-99

[6]

Higher number of Helicobacter pylori CagA EPIYA C phosphorylation sites increases the risk of gastric cancer, but not duodenal ulcer [J].

Batista, Sergio A. ;

Rocha, Gifone A. ;

Rocha, Andreia M. C. ;

Saraiva, Ivan E. B. ;

Cabral, Monica M. D. A. ;

Oliveira, Rodrigo C. ;

Queiroz, Dulciene M. M. .

BMC MICROBIOLOGY, 2011, 11

[7]

cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].

Censini, S ;

Lange, C ;

Xiang, ZY ;

Crabtree, JE ;

Ghiara, P ;

Borodovsky, M ;

Rappuoli, R ;

Covacci, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653

[8]

Coelho Luiz Gonzaga Vaz, 2005, Arq. Gastroenterol., V42, P128

[9]

Detection of the glmM Gene in Helicobacter pylori Isolates with a Novel Primer by PCR [J].

Cordova Espinoza, Maria Guadalupe ;

Gonzalez Vazquez, Rosa ;

Morales Mendez, Iyari ;

Ruelas Vargas, Consuelo ;

Giono Cerezo, Silvia .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1650-1652

[10]

MOLECULAR CHARACTERIZATION OF THE 128-KDA IMMUNODOMINANT ANTIGEN OF HELICOBACTER-PYLORI-ASSOCIATED WITH CYTOTOXICITY AND DUODENAL-ULCER [J].

COVACCI, A ;

CENSINI, S ;

BUGNOLI, M ;

PETRACCA, R ;

BURRONI, D ;

MACCHIA, G ;

MASSONE, A ;

PAPINI, E ;

XIANG, ZY ;

FIGURA, N ;

RAPPUOLI, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5791-5795

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