Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris

被引：57

作者：

Ebrahim, Weaam ^{[1
,2
]}

Kjer, Julia ^{[1
]}

El Amrani, Mustapha ^{[1
]}

Wray, Victor ^{[3
]}

Lin, Wenhan ^{[4
]}

Ebel, Rainer ^{[5
]}

Lai, Daowan ^{[1
]}

Proksch, Peter ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Pharmaceut Biol & Biotechnol, D-40225 Dusseldorf, Germany

[2] Univ Mansoura, Fac Pharm, Dept Pharmacognosy, Mansoura 35516, Egypt

[3] Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany

[4] Peking Univ, Hlth Sci Ctr, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China

[5] Univ Aberdeen, Marine Biodiscovery Ctr, Aberdeen AB24 3UE, Scotland

来源：

MARINE DRUGS | 2012年 / 10卷 / 05期

关键词：

mangrove plants; Sonneratia; endophytes; Bionectria; peptide; structure elucidation; Marfey's method; AMINO-ACIDS; BYSSICOLA; VERTICILLIN; METABOLITES; POLYKETIDE;

D O I：

10.3390/md10051081

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one-and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 mu g/mL.

引用

页码：1081 / 1091

页数：11

共 23 条

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