Homogeneous detection of specific DNA sequences by fluorescence quenching and energy transfer

被引：18

作者：

Morrison, LE ^{[1
]}

机构：

[1] Vysis Inc, Downers Grove, IL 60515 USA

来源：

JOURNAL OF FLUORESCENCE | 1999年 / 9卷 / 03期

关键词：

fluorescence energy transfer; fluorescence quenching; DNA hybridization; homogeneous assays; polymerase chain reaction;

D O I：

10.1023/A:1022551516070

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of fluorescence quenching and energy transfer in DNA hybridization assays is reviewed. Placement of DNA probe labels within interacting distances by hybridization of DNA probes to target DNA or to one another allows rapid homogeneous analysis of specific DNA sequences. Due to the inherently lower sensitivity relative to heterogeneous assays, the fluorescence assays have been coupled with DNA amplification methods such as PCR to provide highly sensitive, clinically relevant homogeneous assays which can be performed in closed systems.

引用

页码：187 / 196

页数：10

共 27 条

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[Anonymous], 1985, RAPID DETECTION IDEN

[2] Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves [J].