Involvement of phosphatidylinositol 3-kinase in nuclear translocation of protein kinase C ζ induced by C2-ceramide in rat hepatocytes

In this study the report that protein kinase C xi (PKC xi), one of the atypical isoforms of the PKC family located predominantly in cytosol, is redistributed by C2-ceramide treatment in isolated hepatocytes. PKC xi increased in membrane and nuclear fractions after 30 min of treatment with C2-ceramide in a dose- and time-dependent manner. The action of C2-ceramide was inhibited by wortmannin and LY 29:14002, indicating that C2-ceramide-induced PKC xiincrease in both nucleus and membrane fractions is mediated by phosphatidylinositol 3-kinase (PI3-kinase) activation. In addition, a significant translocation of PI3-kinase to the nucleus was observed after C2-ceramide treatment. 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.