Antioxidant capacity of human blood plasma and human urine:: Simultaneous evaluation of the ORAC index and ascorbic acid concentration employing pyrogallol red as probe

被引：25

作者：

Torres, P. ^{[2
]}

Galleguillos, P. ^{[1
]}

Lissi, E. ^{[2
]}

Lopez-Alarcon, C. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Chile, Fac Quim, Dept Farm, Santiago 7820436, Chile

[2] Univ Santiago Chile, Fac Quim & Biol, Santiago, Chile

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 20期

关键词：

ORAC index; pyrogallol red; ascorbic acid; human blood plasma; human urine;

D O I：

10.1016/j.bmc.2008.09.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The oxygen radical absorbance capacity ( ORAC) methodology has been employed to estimate the antioxidant capacity of human blood plasma and human urine using pyrogallol red ( ORAC-PGR) as target molecule. Uric acid, reduced glutathione, human serum albumin, and ascorbic acid ( ASC) inhibited the consumption of pyrogallol red, but only ASC generated an induction time. Human blood plasma and human urine protected efficiently pyrogallol red. In these assays, both biological fluids generated neat induction times that were removed by ascorbate oxidase. From these results, ORAC-PGR method could be proposed as a simple alternative to evaluate an ORAC index and, simultaneously, to estimate the concentration of ascorbic acid in human blood plasma or human urine. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：9171 / 9175

页数：5

共 36 条

[1]

Antioxidant capacity of herbal infusions and tea extracts:: A comparison of ORAC-fluorescein and ORAC-pyrogallol red methodologies [J].