A composite chitosan-gelatin bi-layered, biomimetic macroporous scaffold for blood vessel tissue engineering

被引：92

作者：

Badhe, Ravindra V. ^{[1
]}

Bijukumar, Divya ^{[1
]}

Chejara, Dharmesh R. ^{[1
]}

Mabrouk, Mostafa ^{[1
,2
]}

Choonara, Yahya E. ^{[1
]}

Kumar, Pradeep ^{[1
]}

du Toit, Lisa C. ^{[1
]}

Kondiah, Pierre P. D. ^{[1
]}

Pillay, Viness ^{[1
]}

机构：

[1] Univ Witwatersrand, Fac Hlth Sci, Sch Therapeut Sci, Dept Pharm & Pharmacol,Wits Adv Drug Delivery Pla, 7 York Rd, ZA-2193 Parktown, South Africa

[2] Natl Res Ctr, Refractories Ceram & Bldg Mat Dept, 33 El Bohouth St,El-Tahrir St,PO 12622, Giza, Egypt

来源：

CARBOHYDRATE POLYMERS | 2017年 / 157卷

基金：

新加坡国家研究基金会;

关键词：

Bi-layered tubular scaffold; Blood vessel tissue engineering; Chitosan-gelatin composite hydrogel; BIOLOGICAL-PROPERTIES; IN-VITRO; FABRICATION; ARTERIES; POLYMER; DESIGN; CHITIN; DEGRADATION;

D O I：

10.1016/j.carbpol.2016.09.095

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A composite chitosan-gelatin macroporous hydrogel-based scaffold with bi-layered tubular architecture was engineered by solvent casting-co-particulate leaching.The scaffold constituted an inner macroporous layer concealed by a non-porous outer layer mimicking the 3D matrix of blood vessels with cellular adhesion and proliferation. The scaffold was evaluated for its morphological, physicochemical, physicomechanical and biodurability properties employing SEM, FTIR, DSC, XRD, porositometry, rheology and texture analysis. The fluid uptake and biodegradation in the presence of lysozymes was also investigated. Cellular attachment and proliferation was analysed using human dermal fibroblasts (HDF-a) seeded onto the scaffold and evaluated by MTT assay, SEM, and confocal microscopy. Results demonstrated that the scaffold had a desirable tensile strength = 95.81 +/- 11 kPa, elongation at break 112.5 +/- 13%, porosity 82% and pores between 100 and 230 mu m, 50% in vitro biodegradation at day 16 and proliferated fibroblasts over 20 days. These results demonstrate that scaffold may be an excellent tubular archetype for blood vessel tissue engineering. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1215 / 1225

页数：11

共 64 条

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