Mechanisms underlying phasic and sustained secretion in chromaffin cells from mouse adrenal slices

被引:212
作者
Voets, T [1 ]
Neher, E [1 ]
Moser, T [1 ]
机构
[1] Max Planck Inst Biophys Chem, Dept Membrane Biophys, D-37077 Gottingen, Germany
关键词
D O I
10.1016/S0896-6273(00)80812-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Many neurosecretory preparations display two components of depolarization-induced exocytosis: a phasic component synchronized with Ca2+ channel opening, followed by a slower sustained component. We evaluated possible mechanisms underlying this biphasic behavior by stimulating mouse chromaffin cells in situ with both depolarizations and flash photolysis of caged Ca2+. From a direct comparison of the secretory responses to both stimuli, we conclude that phasic and sustained release components originate from a readily releasable pool (RRP) of equally fusion-competent vesicles, suggesting that differences in the vesicles' proximity to Ca2+ channels underlie the biphasic secretory behavior. An intermediate pool in dynamic equilibrium with the RRP ensures rapid recruitment of release-ready vesicles after RRP depletion. Our results are discussed in terms of a refined model for secretion in chromaffin cells.
引用
收藏
页码:607 / 615
页数:9
相关论文
共 42 条
[1]   TIME-COURSE OF CA2+ CONCENTRATION TRIGGERING EXOCYTOSIS IN NEUROENDOCRINE CELLS [J].
CHOW, RH ;
KLINGAUF, J ;
NEHER, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12765-12769
[2]   CO-OPERATIVE ACTION OF CALCIUM IONS IN TRANSMITTER RELEASE AT NEUROMUSCULAR JUNCTION [J].
DODGE, FA ;
RAHAMIMO.R .
JOURNAL OF PHYSIOLOGY-LONDON, 1967, 193 (02) :419-&
[3]  
Fiebig KM, 1999, NAT STRUCT BIOL, V6, P117
[4]   SYNAPTOTAGMIN-I - A MAJOR CA2+ SENSOR FOR TRANSMITTER RELEASE AT A CENTRAL SYNAPSE [J].
GEPPERT, M ;
GODA, Y ;
HAMMER, RE ;
LI, C ;
ROSAHL, TW ;
STEVENS, CF ;
SUDHOF, TC .
CELL, 1994, 79 (04) :717-727
[5]   Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules [J].
Gillis, KD ;
Mossner, R ;
Neher, E .
NEURON, 1996, 16 (06) :1209-1220
[6]   2 COMPONENTS OF TRANSMITTER RELEASE AT A CENTRAL SYNAPSE [J].
GODA, Y ;
STEVENS, CF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12942-12946
[7]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[8]   IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].
HAMILL, OP ;
MARTY, A ;
NEHER, E ;
SAKMANN, B ;
SIGWORTH, FJ .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100
[9]   CALCIUM-DEPENDENCE OF THE RATE OF EXOCYTOSIS IN A SYNAPTIC TERMINAL [J].
HEIDELBERGER, R ;
HEINEMANN, C ;
NEHER, E ;
MATTHEWS, G .
NATURE, 1994, 371 (6497) :513-515
[10]   KINETICS OF THE SECRETORY RESPONSE IN BOVINE CHROMAFFIN CELLS FOLLOWING FLASH-PHOTOLYSIS OF CAGED CA2+ [J].
HEINEMANN, C ;
CHOW, RH ;
NEHER, E ;
ZUCKER, RS .
BIOPHYSICAL JOURNAL, 1994, 67 (06) :2546-2557