cDNA cloning and molecular characterization of mink Smad4

被引：4

作者：

Barnes, E ^{[1
]}

Askham, JM ^{[1
]}

Jones, PE ^{[1
]}

机构：

[1] Univ Leeds, St Jamess Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

来源：

DNA SEQUENCE | 2002年 / 13卷 / 01期

基金：

英国医学研究理事会;

关键词：

Smad4; TGF beta signalling; cDNA cloning; mink;

D O I：

10.1080/10425170290019892

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Smad family of proteins have been implicated as major components of the TGFbeta signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink (Mustela vison) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.

引用

页码：47 / 53

页数：7

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