Molecular cloning and functional expression of the human Golgi UDP-N-acetylglucosamine transporter

被引：64

作者：

Ishida, N

Yoshioka, S

Chiba, Y

Takeuchi, M

Kawakita, M ^{[1
]}

机构：

[1] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1138613, Japan

[2] Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanagawa 2360004, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 1999年 / 126卷 / 01期

关键词：

CDG syndrome; CMP-sialic acid transporter; nucleotide-sugar transporter; UDP-galactose transporter; UDP-N-acetylglucosamine transporter;

D O I：

10.1093/oxfordjournals.jbchem.a022437

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the human UDP-N-acetylglucosamine (UDP-GlcNAc) transporter cDNA, which was recognized through a homology search in the expressed sequence tags database (dbEST) based on its similarity to the human UDP-galactose transporter. The chromosomal location of the UDP-GlcNAc transporter gene was assigned to chromosome 1p21 by fluorescence in situ hybridization (FISH), The transporter was expressed ubiquitously in every tissue so far examined. Expression of the transporter cDNA in CHO-K1 cells in its native and in a C-terminally HA-tagged form indicated that the human UDP-GlcNAc transporter was localized in the Golgi apparatus, The membrane vesicles prepared from yeast cells expressing the cDNA product exhibited UDP-GlcNAc-specific transporting activity. Comparison among UDP-galactose, CMP-sialic acid, and UDP-GlcNAc transporters from several organisms enabled us to identify residues highly conserved among the transporters and residues specific for each group of transporters.

引用

页码：68 / 77

页数：10

共 22 条

[1] Molecular cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter from Kluyveromyces lactis [J].