Acute myeloid leukemia is associated with retroviral gene transfer to hematopoietic progenitor cells in a rhesus macaque

被引：94

作者：

Seggewiss, Ruth

Pittaluga, Stefania

Adler, Rima L.

Guenaga, F. Javier

Ferguson, Cole

Pilz, Ingo H.

Ryu, Byoung

Sorrentino, Brian P.

Young, W. Scott, III

Donahue, Robert E.

von Kalle, Christof

Nienhuis, Arthur W.

Dunbar, Cynthia E.

机构：

[1] NHLBI, Hematol Branch, NIH, DHHS, Bethesda, MD 20892 USA

[2] NCI, Hematopathol Sect, Bethesda, MD 20892 USA

[3] NIMH, Sect Neural Gene Express, NIH, DHHS, Bethesda, MD 20892 USA

[4] St Jude Childrens Hosp, Div Expt Hematol Hematol Oncol, Memphis, TN 38105 USA

[5] Univ Freiburg, Dept Med 1, Freiburg, Germany

[6] Childrens Hosp Res Fdn, Mol & Gene Therapy Program, Cincinnati, OH 45229 USA

来源：

BLOOD | 2006年 / 107卷 / 10期

关键词：

D O I：

10.1182/blood-2005-10-4108

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We report, for the first time, a replication-defective retroviral vector-associated neoplasia in a nonhuman primate. Five years after transplantation with CD34(+) cells transduced with a retroviral vector expressing enhanced green fluorescent protein (eGFP) and a drug-resistant variant of the dihydrofolate reductase gene (L22Y), a rhesus macaque developed a fatal myeloid sarcoma, a type of acute myeloid leukemia. Tumor cells contained 2 clonal vector insertions. One insertion was found in BCL2-A1, an antiapoptotic gene. This event suggests that currently available retroviral vectors may have long-term side effects, particularly in hematopoietic stem and progenitor cells.

引用

页码：3865 / 3867

页数：3

共 16 条

[1]

*BLAT SOFTW U CAL, GEN BROWS

[2] Recurrent retroviral vector integration at the Mds1/Evi1 locus in nonhuman primate hematopoietic cells [J].

Calmels, B ;

Ferguson, C ;

Laukkanen, MO ;

Adler, R ;

Faulhaber, M ;

Kim, HJ ;

Sellers, S ;

Hematti, P ;

Schmidt, M ;

von Kalle, C ;

Akagi, K ;

Donahue, RE ;

Dunbar, CE .

BLOOD, 2005, 106 (07) :2530-2533

[3] Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells [J].

Du, Y ;

Jenkins, NA ;

Copeland, NG .

BLOOD, 2005, 106 (12) :3932-3939

[4] Cooperating cancer-gene identification through oncogenic-retrovirus-induced insertional mutagenesis [J].

Du, Y ;

Spence, SE ;

Jenkins, NA ;

Copeland, NG .

BLOOD, 2005, 106 (07) :2498-2505

[5] A rapid and quantitative assay to estimate gene transfer into retrovirally transduced hematopoietic stem progenitor cells using a 96-well format PCR and fluorescent detection system universal for MMLV-based proviruses [J].

Gerard, CJ ;

Arboleda, MJ ;

Solar, G ;

Mule, JJ ;

Kerr, WG .

HUMAN GENE THERAPY, 1996, 7 (03) :343-354

[6] LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1 [J].

Hacein-Bey-Abina, S ;

Von Kalle, C ;

Schmidt, M ;

McCcormack, MP ;

Wulffraat, N ;

Leboulch, P ;

Lim, A ;

Osborne, CS ;

Pawliuk, R ;

Morillon, E ;

Sorensen, R ;

Forster, A ;

Fraser, P ;

Cohen, JI ;

de Saint Basile, G ;

Alexander, I ;

Wintergerst, U ;

Frebourg, T ;

Aurias, A ;

Stoppa-Lyonnet, D ;

Romana, S ;

Radford-Weiss, I ;

Gross, F ;

Valensi, F ;

Delabesse, E ;

Macintyre, E ;

Sigaux, F ;

Soulier, J ;

Leiva, LE ;

Wissler, M ;

Prinz, C ;

Rabbitts, TH ;

Le Deist, F ;

Fischer, A ;

Cavazzana-Calvo, M .

SCIENCE, 2003, 302 (5644) :415-419

[7] Distinct genomic integration of MLV and SIV vectors in primate hematopoietic stem and progenitor cells [J].

Hematti, P ;

Hong, BK ;

Ferguson, C ;

Adler, R ;

Hanawa, H ;

Sellers, S ;

Holt, IE ;

Eckfeldt, CE ;

Sharma, Y ;

Schmidt, M ;

von Kalle, C ;

Persons, DA ;

Billings, EM ;

Verfaillie, CM ;

Nienhuis, AW ;

Wolfsberg, TG ;

Dunbar, CE ;

Calmels, B .

PLOS BIOLOGY, 2004, 2 (12) :2183-2190

[8] Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein [J].

Kelly, PF ;

Vandergriff, J ;

Nathwani, A ;

Nienhuis, AW ;

Vanin, EF .

BLOOD, 2000, 96 (04) :1206-1214

[9] Prolonged multilineage clonal hematopoiesis in a rhesus recipient of CD34 positive cells marked with a RD114 pseudotyped oncoretroviral vector [J].

Kelly, PF ;

Donahue, RE ;

Vandergriff, JA ;

Takatoku, M ;

Bonifacino, AC ;

Agricola, BA ;

Metzger, ME ;

Dunbar, CE ;

Nienhuis, AW ;

Vanin, EF .

BLOOD CELLS MOLECULES AND DISEASES, 2003, 30 (01) :132-143

[10] The human genome browser at UCSC [J].

Kent, WJ ;

Sugnet, CW ;

Furey, TS ;

Roskin, KM ;

Pringle, TH ;

Zahler, AM ;

Haussler, D .

GENOME RESEARCH, 2002, 12 (06) :996-1006

← 1 2 →