The binding profile of Actinobacillus pleuropneumoniae serotypes 1 and 2 to various glycosphingolipids was evaluated by using thin-layer chromatogram overlay. A. pleuropneumoniae whole cells recognized glucosylceramide (Glc beta 1Cer), galactosylceramide (Gal beta 1Cer) with hydroxy and nonhydroxy fatty acids, sulfatide (SO(3)-Gal beta 1Cer), lactosylceramide (Gal beta 1-4Glc beta 1Cer), gangliotriaosylceramide GgO(3) (GalNAc beta 1-4Gal beta 1-4Glc beta 1Cer), and gangliotetraosylceramide GgO(4) (Gal beta 1-3GalNAc beta 1-4Gal beta 1-4Glc beta 1Cer) glycosphingolipids. We observed no binding to globoseries, globotriaosylceramide Gb(3), globoside Gb(4), or Forssman Gb(5) glycosphingolipids or to gangliosides GM1, GM2, GM3, GD1a, GD1b, GD3, and GT1b, The A. pleuropneumoniae strains tested also failed to detect phosphatidylethanolamine or ceramide, Interestingly, extracted lipopolysaccharide (LPS) of serotype 1 and serotype 2 as well as detoxified LPS of serotype 1 showed binding patterns similar to that of whole bacterial cells. Binding to GlcCer, GalCer, sulfatide, and LacCer, but not to GgO(3) and GgO(4) glycosphingolipids, was inhibited after incubation of the bacteria with monoclonal antibodies against LPS O antigen. These findings indicate the involvement of LPS in recognition of three groups of glycosphingolipids: (i) GlcCer and LacCer, where glucose is probably an important saccharide sequence required for LPS binding; (ii) GalCer and sulfatide glycosphingolipids, where the sulfate group is part of the binding epitope of the isoreceptor; and (iii) GgO(3) and GgO(4), where GalNac beta 1-4Gal disaccharide represents the minimal common binding epitope, Taken together, our results indicate that A, pleuropneumoniae LPS recognize various saccharide sequences found in different glycosphingolipids, which probably represents a strong virulence attribute.