Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods

被引:74
作者
Leclercq, A
Wanegue, C
Baylac, P
机构
[1] Inst Pasteur Lille, Pole Sante Aliment Nutr, F-59651 Villeneuve Dascq, France
[2] Commissariat Armee Terre, Serv Cent Etudes & Realisat, Dept Microbiol Alimentaire, F-00447 Armees, France
关键词
D O I
10.1128/AEM.68.4.1631-1638.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1degreesC and transfer to 44 +/- 1degreesC for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about I log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.
引用
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页码:1631 / 1638
页数:8
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