Rotation of the gamma subunit in F-1-ATPase; Evidence that ATP synthase is a rotary motor enzyme

被引：27

作者：

Yasuda, R

Noji, H

Kinosita, K

Motojima, F

Yoshida, M

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA,KANAGAWA 226,JAPAN

[2] KEIO UNIV,FAC SCI & TECHNOL,DEPT PHYS,KOHOKU KU,YOKOHAMA,KANAGAWA 223,JAPAN

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 1997年 / 29卷 / 03期

关键词：

ATP synthase; F-1-ATPase; rotary motor; single molecule imaging; loose coupling;

D O I：

10.1023/A:1022449708449

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

ATP-dependent, azide-sensitive rotation of the gamma subunit relative to the alpha(3) beta(3) hexagonal ring of ATP synthase was observed with a single molecule imaging system. Thus, ATP synthase is a rotary motor enzyme, the first ever found.

引用

页码：207 / 209

页数：3

共 8 条

[1] STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF F1-ATPASE FROM BOVINE HEART-MITOCHONDRIA [J].

ABRAHAMS, JP ;

LESLIE, AGW ;

LUTTER, R ;

WALKER, JE .

NATURE, 1994, 370 (6491) :621-628

[2]

BOYER PD, 1997, IN PRESS ANN REV BIO, V66

[3] ROTATION OF SUBUNITS DURING CATALYSIS BY ESCHERICHIA-COLI F1-ATPASE [J].

DUNCAN, TM ;

BULYGIN, VV ;

ZHOU, Y ;

HUTCHEON, ML ;

CROSS, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :10964-10968

[4] LIGAND-DEPENDENT STRUCTURAL VARIATIONS IN ESCHERICHIA-COLI-F1 ATPASE REVEALED BY CRYOELECTRON MICROSCOPY [J].

GOGOL, EP ;

JOHNSTON, E ;

AGGELER, R ;

CAPALDI, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) :9585-9589

[5]

GRESSER MJ, 1982, J BIOL CHEM, V257, P2030

[6] EXPRESSION OF THE WILD-TYPE AND THE CYS-/TRP-LESS ALPHA(3)BETA(3)GAMMA COMPLEX OF THERMOPHILIC F1-ATPASE IN ESCHERICHIA-COLI [J].

MATSUI, T ;

YOSHIDA, M .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1995, 1231 (02) :139-146

[7] Direct observation of the rotation of F-1-ATPase [J].

Noji, H ;

Yasuda, R ;

Yoshida, M ;

Kinosita, K .

NATURE, 1997, 386 (6622) :299-302

[8] Intersubunit rotation in active F-ATPase [J].

Sabbert, D ;

Engelbrecht, S ;

Junge, W .

NATURE, 1996, 381 (6583) :623-625

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