Detection of exogenous genes in genetically modified plants with multiplex polymerase chain reaction

被引:14
作者
Tao, Z [1 ]
Cai, XF [1 ]
Yang, SL [1 ]
Gong, Y [1 ]
机构
[1] Chinese Acad Sci, SIBS, Shanghai Res Ctr Biotechnol, Beijing 100864, Peoples R China
关键词
genetically modified plants; multiplex PCR; PBI121; soybean; tobacco;
D O I
10.1007/BF02772827
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To detect exogenous genes in genetically modified plants, we have designed a multiplex polymerase chain reaction (MPCR) system that includes 7 primer pairs. With this method, the promoter, terminator, selection gene, reporter gene, and function gene can be detected simultaneously. The concentrations of each primer were optimized according to AT/TA%. Several genes in plasmid PB1121, genetically modified tobacco, and imported soybeans were detected, and the results were consistent with standard PCR. The method was sensitive enough to detect a single copy sequence.
引用
收藏
页码:289 / 298
页数:10
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