Detection of exogenous genes in genetically modified plants with multiplex polymerase chain reaction

被引：14

作者：

Tao, Z ^{[1
]}

Cai, XF ^{[1
]}

Yang, SL ^{[1
]}

Gong, Y ^{[1
]}

机构：

[1] Chinese Acad Sci, SIBS, Shanghai Res Ctr Biotechnol, Beijing 100864, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2001年 / 19卷 / 04期

关键词：

genetically modified plants; multiplex PCR; PBI121; soybean; tobacco;

D O I：

10.1007/BF02772827

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To detect exogenous genes in genetically modified plants, we have designed a multiplex polymerase chain reaction (MPCR) system that includes 7 primer pairs. With this method, the promoter, terminator, selection gene, reporter gene, and function gene can be detected simultaneously. The concentrations of each primer were optimized according to AT/TA%. Several genes in plasmid PB1121, genetically modified tobacco, and imported soybeans were detected, and the results were consistent with standard PCR. The method was sensitive enough to detect a single copy sequence.

引用

页码：289 / 298

页数：10

共 26 条

[1] MULTIPLEX PCR AMPLIFICATION AND IMMOBILIZED CAPTURE PROBES FOR DETECTION OF BACTERIAL PATHOGENS AND INDICATORS IN WATER [J].

BEJ, AK ;

MAHBUBANI, MH ;

MILLER, R ;

DICESARE, JL ;

HAFF, L ;

ATLAS, RM .

MOLECULAR AND CELLULAR PROBES, 1990, 4 (05) :353-365

[2] DELETION SCREENING OF THE DUCHENNE MUSCULAR-DYSTROPHY LOCUS VIA MULTIPLEX DNA AMPLIFICATION [J].

CHAMBERLAIN, JS ;

GIBBS, RA ;

RANIER, JE ;

NGUYEN, PN ;

CASKEY, CT .

NUCLEIC ACIDS RESEARCH, 1988, 16 (23) :11141-11156

[3] Hazardous CaMV promoter? [J].

Joe Cummins ;

Mae-Wan Ho ;

Angela Ryan .

Nature Biotechnology, 2000, 18 (4) :363-363

[4] IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY [J].

FREIER, SM ;

KIERZEK, R ;

JAEGER, JA ;

SUGIMOTO, N ;

CARUTHERS, MH ;

NEILSON, T ;

TURNER, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9373-9377

[5] Agbiotech and world food security - threat or boon? [J].

Gilmore, R .

NATURE BIOTECHNOLOGY, 2000, 18 (04) :361-361

[6]

GU HY, 1995, PLANT GENES MOL MANI

[7] Scientists avert new GMO crisis [J].

Hodgson, J .

NATURE BIOTECHNOLOGY, 2000, 18 (01) :13-13

[8]

JAMES C, 2000, SHENG WU JI SHU TONG, V3, P39

[9] A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay [J].

James, D .

JOURNAL OF VIROLOGICAL METHODS, 1999, 83 (1-2) :1-9

[10]

JIA SR, 1997, SHENG WU JI SHU TONG, V1, P4

← 1 2 3 →