The comparison of proteins with immunochemical affinity to stress protein 310 kD in cytoplasmatic proteins of winter rye, winter wheat, Elymus and maize

The search for proteins, immunochemically related to winter rye CSP 310 among the native cytoplasmatic proteins of a number of cultivated cereals with different tolerance to low temperatures-maize, winter wheat and winter rye and the very low temperature tolerant wild grass-Elymus sibiricus was carried out. Western blotting showed that among the native cytoplasmatic proteins of all species investigated there are proteins immunochemically related to CSP 310 protein with molecular weights about 230 and about 140-110 kD. Proteins with molecular weights about 480 and 310 kD were found in significant amounts only in winter rye. In E. sibiricus proteins with molecular weights 380-320 kD were present but these were not present among the cytoplasmatic protein spectra of the other species. In each case the proteins immunochemically related to CSP 310 consisted of different combinations of two types of subunits. (C) 1999 Elsevier Science Ltd. All rights reserved.