Increased interleukin-4 production by NK T cells in systemic lupus erythematosus

被引:25
作者
Funauchi, M [1 ]
Yu, H [1 ]
Sugiyama, M [1 ]
Ikoma, S [1 ]
Ohno, M [1 ]
Kinoshita, K [1 ]
Hamada, K [1 ]
Kanamaru, A [1 ]
机构
[1] Kinki Univ, Sch Med, Dept Internal Med 3, Osaka 5898511, Japan
关键词
systemic lupus erythematosus; NK T cells; interleukin-4;
D O I
10.1006/clim.1999.4742
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
It has been reported that production of interleukin (IL)-4, a T helper (Th)-2-type cytokine, might play an important role in the pathogenesis of systemic lupus erythematosus (SLE). On the other hand, it is known that NK1.1(+) cells which belong to CD4, CD8 double-negative, or CD4(+) cells are associated with initial IL-4 production and Th2 differentiation in mice although human equivalent cells are unknown. In order to study the profile of IL-4-producing cells in SLE, cytoplasmic IL-4 and various surface antigens on peripheral mononuclear cells were analyzed. Peripheral mononuclear cells were stimulated for 5 h by phorbol ester and ionomycin in the presence of monensin, fixed, and permeabilized with paraformaldehyde and saponin solution. Then cytoplasmic IL-4 and various surface antigens were analyzed by flow cytometry. IL-4-producing cells in SLE were phenotypically the same as those which produce IL-4 normally and frequently bore activated T-cell (CD7, CD25, CD28, CD29) and NK-cell markers (CD56, CD57). Double-negative T cells and CD57(+) T cells were increased in number and were more frequently positive for cytoplasmic IL-4 in SLE compared with normal controls and Various infectious diseases. It was suggested that T cells with NK cell markers, CD57(+) T cells, which are known to extrathymically differentiate, might be involved in the pathogenesis of SLE as a counterpart of mouse NK1.1(+) cells. (C) 1999 Academic Press.
引用
收藏
页码:197 / 202
页数:6
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