Genetic analysis of the complete gag and env genes of HIV type 1 subtype C primary isolates from South Africa

被引：21

作者：

Engelbrecht, S

de Villiers, T

Sampson, CC

Megede, JZ

Barnett, SW

van Rensburg, EJ

机构：

[1] Univ Stellenbosch, Dept Med Virol, ZA-7505 Tygerberg, South Africa

[2] Tygerberg Hosp, ZA-7505 Tygerberg, South Africa

[3] Chiron Corp, Emeryville, CA 94608 USA

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 2001年 / 17卷 / 16期

关键词：

D O I：

10.1089/08892220152644241

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

South Africa has one of the fastest growing HIV-1 epidemics, with an estimated 4.7 million people infected. To better understand the genetic diversity of this epidemic and its potential impact on vaccine development, we have cloned and sequenced the complete gag and env genes of 13 primary virus isolates. Phylogenetic analysis of our sequences and 69 complete env genes from the Los Alamos and GenBank databases revealed multiple subclusters within subtype C. The V3 loop region was relatively conserved in all our strains when compared with other subtypes, but the region immediately downstream was highly variable. No intersubtype recombinant forms were observed when comparing the gag and env sequences. Characterization of the complete gag and env genes enabled us to select specific strains for further vaccine development.

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页码：1533 / 1547

页数：15

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