JAK/STAT signalling in Drosophila controls cell motility during germ cell migration

被引:31
作者
Brown, S
Zeidler, MP
Hombría, JECG
机构
[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England
[2] Max Planck Inst Biophys Chem, Abt Mol Entwicklungsbiol, D-37077 Gottingen, Germany
[3] Univ Pablo Olavide, CABD, CSIC, Seville, Spain
基金
英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金;
关键词
JAK/STAT; pole cells; Drosophila; primordial germ cells; gonad; migration; cell survival; filopodia;
D O I
10.1002/dvdy.20709
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The gonad is formed from two populations of cells originating at different locations: the primordial germ cells (PGCs), giving rise to either sperm or oocytes, and the somatic gonadal mesoderm precursors (SGPs), which support development of the gametes. Following the PGCs' migration during gastrulation, these two populations meet, forming the immature gonad. We present evidence that during embryonic development, the PGCs require the canonical JAK/STAT signalling cascade to migrate efficiently towards the SGPs. Loss of function for any element of the JAK/STAT pathway causes frequent germ cell mislocalisation. We have found that wild-type germ cells produce filopodia while they migrate through the mesoderm towards the gonad. Our observations suggest that PGCs use filopodia to migrate and to keep contact with each other. Interestingly, activation of the JAK/STAT pathway is required for these filopodia to form, and ectopic JAK/STAT activation enhances their formation.
引用
收藏
页码:958 / 966
页数:9
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