Sevoflurane reduces myocardial infarct size and decreases the time threshold for ischemic preconditioning in dogs

被引:140
作者
Toller, WG
Kersten, JR
Pagel, PS
Hettrick, DA
Warltier, DC
机构
[1] Med Coll Wisconsin, Dept Anesthesiol, Milwaukee, WI 53226 USA
[2] Med Coll Wisconsin, Dept Pharmacol & Toxicol, Milwaukee, WI 53226 USA
[3] Med Coll Wisconsin, Dept Med, Milwaukee, WI 53226 USA
[4] Zablocki Vet Adm Med Ctr, Milwaukee, WI 53295 USA
关键词
glyburide; myocardial infarction; volatile anesthetics;
D O I
10.1097/00000542-199911000-00037
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Background: Recent evidence indicates that volatile anesthetics exert protective effects during myocardial ischemia and reperfusion. The authors tested the hypothesis that sevoflurane decreases myocardial infarct size by activating adenosine triphosphate-sensitive potassium (K-ATP) channels and reduces the time threshold of ischemic preconditioning necessary to protect against infarction. Methods: Barbiturate-anesthetized dogs (n = 75) were instrumented for measurement of aortic and left ventricular pressures and maximum rate of increase of left ventricular pressure and mere subjected to a 60-min left anterior descending (LAD) coronary artery occlusion followed by 3-h reperfusion. In four separate groups, dogs received vehicle or the K-ATP channel antagonist glyburide (0.1 mg/kg intravenously), and 1 minimum alveolar concentration sevoflurane (administered until immediately before coronary artery occlusion) in the presence or absence of glyburide. In three additional experimental groups, sevoflurane was discontinued 30 min (memory) before the 60-min LAD occlusion or a 2-min LAD occlusion as an ischemic preconditioning stimulus was used with or without subsequent sevoflurane (with memory) pretreatment. Regional myocardial perfusion and infarct size were measured with radioactive microspheres and triphenyltetrazolium staining, respectively. Results: Vehicle (23 +/- 1% of the area at risk; mean +/- SEM) and glyburide (25 +/- 2%) alone produced equivalent effects on myocardial infarct size. Sevoflurane significantly (P < 0.05) decreased infarct size (13 +/- 2%). This beneficial effect was abolished by glyburide (21 +/- 3%). Neither the 2-min LAD occlusion nor sevoflurane followed by 30 min of memory were protective alone, but together, sevoflurane enhanced the effects of the brief ischemic stimulus and profoundly reduced infarct size (9 +/- 2%). Conclusion: Sevoflurane reduces myocardial infarct size by activating K-ATP channels and reduces the time threshold for ischemic preconditioning independent of hemodynamic effects is vivo.
引用
收藏
页码:1437 / 1446
页数:10
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