Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles

被引：181

作者：

Li, ZP ^{[1
]}

Duan, XR ^{[1
]}

Liu, CH ^{[1
]}

Du, BA ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Hebei Province, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2006年 / 351卷 / 01期

基金：

中国国家自然科学基金;

关键词：

resouance light scattering; gold nanoparticles; cysteine;

D O I：

10.1016/j.ab.2006.01.038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between cysteine and gold nanoparticles was studied. Through the covalent combination with the -SH group and the electrostatic binding with the -NH3+ group of cysteine, gold nanoparticles call self-assemble to form a network Structure, Which results in greatly enhanced resonance light scattering (RLS). The experimental results demonstrate that the RLS technique offers a sensitive tool for investigations of self-assembly of nanoparticles. On the other hand, the RLS method can be applied to selectively determine cysteine with high sensitivity and simple operation. The linear range of determination of cysteine is from 0.01 to 0.25 mu g/mL with the detection limit of 2.0 ng/mL (16.5 nM, 3 sigma). None of the amino acids found in proteins inteferes with the determination. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：18 / 25

页数：8

共 22 条

[1] Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes [J].

Authier, L ;

Grossiord, C ;

Brossier, P ;

Limoges, B .

ANALYTICAL CHEMISTRY, 2001, 73 (18) :4450-4456

[2] Nile red-adsorbed gold nanoparticles for selective determination of thiols based on energy transfer and aggregation [J].

Chen, SJ ;

Chang, HT .

ANALYTICAL CHEMISTRY, 2004, 76 (13) :3727-3734

[3] Determination of cysteine in human plasma by high-performance liquid chromatography and ultraviolet detection after pre-column derivatization with 2-chloro-1-methylpyridinium iodide [J].

Chwatko, G ;

Bald, E .

TALANTA, 2000, 52 (03) :509-515

[4] Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].

Elghanian, R ;

Storhoff, JJ ;

Mucic, RC ;

Letsinger, RL ;

Mirkin, CA .

SCIENCE, 1997, 277 (5329) :1078-1081

[5] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[6] PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].

GRABAR, KC ;

FREEMAN, RG ;

HOMMER, MB ;

NATAN, MJ .

ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743

[7] Determination of cysteine by capillary zone electrophoresis with end-column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation [J].

Jin, WR ;

Wang, Y .

JOURNAL OF CHROMATOGRAPHY A, 1997, 769 (02) :307-314

[8] OPTICAL-ABSORPTION OF SMALL METALLIC PARTICLES [J].

KREIBIG, U ;

GENZEL, L .

SURFACE SCIENCE, 1985, 156 (JUN) :678-700

[9] Determination of cysteine in a pharmaceutical formulation by flow injection analysis with a chemiluminescence detector [J].

Lau, CW ;

Qin, XJ ;

Liang, HY ;

Lu, HZ .

ANALYTICA CHIMICA ACTA, 2004, 514 (01) :45-49

[10] Bio-barcodes based on oligonucleotide-modified nanoparticles [J].

Nam, JM ;

Park, SJ ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (15) :3820-3821

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