Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC/MS in the NICI mode

被引：23

作者：

Goulitquer, Sophie ^{[1
,2
]}

Dreano, Yvonne ^{[1
,2
]}

Berthou, Francois ^{[1
,2
,3
]}

Corcos, Laurent ^{[1
,2
,4
]}

Lucas, Daniele ^{[1
,2
,3
]}

机构：

[1] Univ Europeenne Bretagne, Fac Med, F-29200 Brest, France

[2] Univ Brest, INSERM, ECLA, U613, F-29200 Brest, France

[3] CHU Brest, Biochim Lab, F-29200 Brest, France

[4] Etab Francais Sang Bretagne, F-29200 Brest, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 876卷 / 01期

关键词：

Epoxyeicosatrienoic acids; Eicosanoids; Cytochrome P450; Human red blood cells; GC/MS/NICI;

D O I：

10.1016/j.jchromb.2008.10.035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid involved in the regulation of vascular tone. Despite the importance of EETs in a variety of physiological effects, few methods have been developed to quantity them in human blood. This led us to develop a method by GC/MS with negative ion chemical ionization. As EETs are primarily located in phospholipids, red blood cells (RBCs) and plasma phospholipids were hydrolyzed with phospholipase A(2) after a solid phase extraction. Then, EETs were derivatized as pentafluorobenzyl esters, and [H-2(8)]-arachidonic acid was used as internal standard for quantification. EETs were found to be at concentrations of 106 +/- 37 ng mL(-1) in plasma and 33.4 +/- 8.5 ng/10(9) RBCs (mean +/- S.D.) in 10 healthy volunteers. Their amount in RBCs was 3-fold that in plasma: both parameters proved to be well correlated. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：83 / 88

页数：6

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