Anti-inflammatory and antinociceptive effects of Chinese medicine SQ gout capsules and its modulation of pro-inflammatory cytokines focusing on gout arthritis

被引：55

作者：

Kodithuwakku, Nandani Darshika ^{[1
]}

Pan, Min ^{[1
]}

Zhu, Yi-lin ^{[1
]}

Zhang, Yan-yan ^{[1
]}

Feng, Yi-dong ^{[1
]}

Fang, Wei-rong ^{[1
]}

Li, Yun-man ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Physiol, State Key Lab Med, Nanjing 210009, Jiangsu, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2013年 / 150卷 / 03期

关键词：

Anti-inflammation; Antinociceptive effect; SQ gout capsule; Gout arthritis; Pro-inflammatory cytokines; CRYSTAL-INDUCED INFLAMMATION; NECROSIS-FACTOR-ALPHA; HERBAL MEDICINE; ACUTE TOXICITY; MECHANISMS; MICE; INHIBITION; ACTIVATION; RATS; HYPERURICEMIA;

D O I：

10.1016/j.jep.2013.10.016

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: Shuang-Qi gout capsule is a traditional Chinese medicine prescription, which has been used in the treatment of joint pain, inflammation and gout arthritis. This study evaluates anti-inflammatory and antinociceptive effects of Shuang-Qi gout capsule and its modulation of pro-inflammatory cytokines with special reference to gout arthritis. Materials and methods: Anti-inflammatory effect of Shuang-Qi gout capsule was investigated bymice tail-flick response, acetic acid induced writhing response, Xylene-induced auricle inflammation and the hind paw volume of the monosodium urate (MSU) crystal induced rats with different time durations. To investigate the effects on gout arthritis, ankle joint of rats induced by MSU crystals and assessed for edema and histopathological changes. In vitro, prepared serum was incubated with urate crystal induced HUVE cells and the release of TNF-alpha and IL-1 beta determined by ELISA. Results: Shuang-Qi gout capsule showed significant and dose dependent anti-inflammatory effect via reducing edema and pain, throughout all the models. The high dose of Shuang-Qi gout capsule and Indomethacin significantly attenuated the edema. Histopathological results showed that high and medium dose of Shuang-Qi gout capsule and Indomethacin reduced gouty joint inflammatory features, while the high dose of Shuang-Qi gout capsule showed a better therapeutic effect. High and medium dose of Shuang-Qi gout capsule significantly reduced the release of TNF-alpha and IL-1 beta (p < 0.05). Conclusion: Shuang-Qi gout capsule can effectively inhibit the inflammation, analgesia, through the modulation of emission of pro-inflammatory cytokines and the curative effect is dose dependent. Conversely, these MSU induced in vivo and in vitro studies of Shuang-Qi gout capsule suggest that, Shuang-Qi gout capsule may be a potential agent for treatment in gouty arthritis. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1071 / 1079

页数：9

共 47 条

[1]

EVIDENCE FOR AN ACTION OF MORPHINE AND THE ENKEPHALINS ON SENSORY NERVE-ENDINGS IN THE MOUSE PERITONEUM [J].