Use of brain slices in the study of pathogenic role of inducible nitric oxide synthase in cerebral ischemia-reperfusion

被引：15

作者：

De Alba, J ^{[1
]}

Cárdenas, A ^{[1
]}

Moro, MA ^{[1
]}

Leza, JC ^{[1
]}

Lorenzo, P ^{[1
]}

Lizasoain, I ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Med, Dept Farmacol, E-28040 Madrid, Spain

来源：

GENERAL PHARMACOLOGY | 1999年 / 32卷 / 05期

关键词：

cerebral ischemia; hypoxia; lactate dehydrogenase; neuronal death; neuron-specific enolase; nitric oxide;

D O I：

10.1016/S0306-3623(98)00280-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We have recently demonstrated that inducible nitric oxide synthase (iNOS) is expressed in rat forebrain slices exposed to oxygen and glucose deprivation (OGD). Now, we have found that the expression of iNOS after OGD is time-dependent since 20 min of OGD produces the appearance of iNOS at earlier times than 10 min of OGD. OGD also causes neurotoxicity in this model, as revealed by the increase in excitatory amino acid, neuron specific enolase and lactate dehydrogenase (LDH) efflux to the incubation solution. Finally, the administration of the NMDA receptor antagonist MK-801 (100 nM) inhibits both the expression of iNOS and the release of LDH. Our findings demonstrate that this method may be considered an useful in vitro model of ischemia-reperfusion to determine the therapeutic role of neuroprotective tools. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：577 / 581

页数：5

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