Overexpression of carboxylesterase-1 and mutation (F439H) of acetylcholinesterase-1 are associated with chlorpyrifos resistance in Laodelphax striatellus

被引：36

作者：

Zhang, Yueliang ^{[1
]}

Li, Shuo ^{[1
]}

Xu, Lu ^{[2
]}

Guo, Hui Fang ^{[1
]}

Zi, Jinyan ^{[1
,2
]}

Wang, Lihua ^{[1
]}

He, Peng ^{[3
]}

Fang, Jichao ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing, Jiangsu, Peoples R China

[3] Guizhou Univ, Minist Educ, Key Lab Green Pesticide & Agr Bioengn, State Key Lab Breeding Base Green Pesticide & Agr, Guiyang 550003, Peoples R China

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2013年 / 106卷 / 1-2期

关键词：

Laodelphax striatellus; Insecticide resistance; Carboxylesterase; Protein quantity; Acetylcholinesterase; Mutation frequency; AMINO-ACID SUBSTITUTION; COLORADO-POTATO BEETLE; INSECTICIDE RESISTANCE; ORGANOPHOSPHATE RESISTANCE; POSSIBLE MECHANISMS; FIELD POPULATIONS; FALLEN HOMOPTERA; MYZUS-PERSICAE; MESSENGER-RNA; EXPRESSION;

D O I：

10.1016/j.pestbp.2013.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies investigating a chlorpyrifos-resistant (YN-CPF) strain indicated chlorpyrifos resistance was a result of Laodelphax striatellus carboxylesterase-1 (Ls.CarE1)-mediated detoxification and acetylcholinesterase (AChE) insensitivity. In this study, we found via immunoblotting that the protein level of Ls.CarE1 was significantly overexpressed in the YN-CPF strain compared with that observed in the chlorpyrifos-susceptible (YN) strain. Moreover, the full-length gene encoding acetylcholinesterase-1, designated Ls.AChE1, was cloned from the YN-CPF and YN strains. A sequence analysis found an amino acid mutation (Phe439His) in Ls.AChE1 (corresponding to F331 in the standard matured AChE sequence of Torpedo califomica) and the mutation frequencies of Phe439His were 91%, 54% and 0% in the YN-CPF, the chlorpyrifos relaxed selection (YN-RSF) and the YN strains, respectively. The results offer new evidence that support the involvement of both AChE insensitivity and CarE metabolism in the resistance to chlorpyrifos in the YN-CPF strain. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：8 / 13

页数：6

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