Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste

被引：78

作者：

Muhr, Alexander ^{[1
]}

Rechberger, Eva Maria ^{[1
]}

Salerno, Anna ^{[1
]}

Reiterer, Angelika ^{[1
]}

Malli, Karin ^{[1
]}

Strohmeier, Katharina ^{[2
]}

Schober, Sigurd ^{[2
]}

Mittelbach, Martin ^{[2
]}

Koller, Martin ^{[1
,3
]}

机构：

[1] Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria

[2] Graz Univ, Inst Chem, A-8010 Graz, Austria

[3] ARENA Assoc Resource Efficient & Sustainable Tech, A-8010 Graz, Austria

来源：

JOURNAL OF BIOTECHNOLOGY | 2013年 / 165卷 / 01期

关键词：

Biodegradable resin; Biodiesel; mcl-PHA; Polyhydroxyalkanoate; Pseudomonas chlororaphis; MEDIUM-CHAIN-LENGTH; RALSTONIA-EUTROPHA; GENOME SEQUENCE; CULTURE SYSTEM; POLYHYDROXYALKANOATES; PUTIDA; POLY(HYDROXYALKANOATES); BACTERIA; BATCH; ACID;

D O I：

10.1016/j.jbiotec.2013.02.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (mu(max.)) of 0.08 h(-1), 0.10 h(-1) and 0.13 h(-1), respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum. (C) 2013 Elsevier B. V. All rights reserved.

引用

页码：45 / 51

页数：7

共 54 条

[1] Polyhydroxyalkanoate (PHA) production by a mixed microbial culture using sugar molasses: Effect of the influent substrate concentration on culture selection [J].