A one-pot total synthesis of crambin

被引：289

作者：

Bang, D ^{[1
]}

Kent, SBH ^{[1
]}

机构：

[1] Univ Chicago, Inst Biophys Dynam, Dept Biochem & Mol Biol, Dept Chem, Chicago, IL 60637 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 19期

关键词：

crambin; native chemical ligation; protein engineering; protein folding;

D O I：

10.1002/anie.200353540

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40%. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.

引用

页码：2534 / 2538

页数：5

共 17 条

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