An improved method for rapid analysis of the fatty acids of glycerolipids

被引：388

作者：

Ichihara, K

Shibahara, A

Yamamoto, K

Nakayama, T

机构：

[1] OSAKA PREFECTURAL COLL HLTH SCI,HABIKINO 583,JAPAN

[2] JAPAN OIL STUFF INSPECTORS CORP,GEN TESTING RES INST,KOBE,HYOGO 658,JAPAN

来源：

LIPIDS | 1996年 / 31卷 / 05期

关键词：

D O I：

10.1007/BF02522648

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20-40 mg) were mixed with 2 mt of hexane and 0.2 mt of 2 M methanolic KOH at room temperature for 1-2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240 degrees C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed within 5 min for common vegetable oils and 8 min for fish oils.

引用

页码：535 / 539

页数：5

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