Molecular cloning and expression analysis of three genes encoding starch synthase II in rice

被引：60

作者：

Jiang, HW ^{[1
]}

Dian, WM ^{[1
]}

Liu, FY ^{[1
]}

Wu, P ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310029, Peoples R China

来源：

PLANTA | 2004年 / 218卷 / 06期

基金：

中国国家自然科学基金;

关键词：

evolution; gelatinization property; gene expression; Oryza; starch synthase;

D O I：

10.1007/s00425-003-1189-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Three starch synthase (SS) genes, OsSSII-1, OsSSII-2 and OsSSII-3, were identified in rice (Oryza sativa L.) and localized to chromosomes 10, 2 and 6, respectively. The three OsSSII full-length cDNAs were cloned, and the predicted amino acid sequences were found to share 52-73% similarity with other members of the plant SSII family. The SS activity of each OsSSII was confirmed by expression and enzyme activity assay in Escherichia coli. Expression profile analysis revealed that OsSSII-1 was expressed in endosperms, leaves and roots; OsSSII-2 was mainly expressed in leaves, while OsSSII-3 was mainly expressed in endosperms. Similar to the OsSSI proteins, the OsSSII-2 and OsSSII-3 proteins were found in the soluble as well as the starch-granule-bound fractions in rice. The roles of the OsSSII proteins in starch biosynthesis in rice and the evolutionary relationships of the genes encoding monocotyledonous and dicotyledonous class-II SS enzymes are discussed.

引用

页码：1062 / 1070

页数：9

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