Helicobacter pylori interactions with human gastric mucin studied with a resonant mirror biosensor

被引:25
作者
Hirmo, S
Artursson, E
Puu, G
Wadström, T
Nilsson, B [1 ]
机构
[1] Def Res Estab, Dept NBC Def, S-90182 Umea, Sweden
[2] Lund Univ, Inst Med Microbiol, S-22362 Lund, Sweden
关键词
Helicobacter pylori; resonant mirror biosensor; gastric mucin;
D O I
10.1016/S0167-7012(99)00060-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Helicobacter pylori, a human pathogen colonizing the gastrointestinal tract, is first interacting with mucus glycoproteins to penetrate the gastric mucus layer and then attach to specific epithelial cell targets. An optical biosensor technique based on the resonant mirror was used to study H. pylori interactions with human gastric mucin. The mucin preparation was immobilized on the sensor surface to set up the experimental model, close to the in vivo situation. Both sialylated and sulphated oligosaccharides interfered with the H. pylori binding to the immobilized mucin by reducing or abolishing the binding of the bacteria. Furthermore, the displacement of the bacteria from immobilized mucin by highly sulphated glycosaminoglycans was observed. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:177 / 182
页数:6
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