RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity

被引：60

作者：

Choi, Won Sup ^{[1
]}

Bae, Jin Woo ^{[1
]}

Lim, Hye Ryeon ^{[2
]}

Joung, Yoon Ki ^{[1
]}

Park, Jong-Chul ^{[2
]}

Kwon, Il Keun ^{[3
,4
]}

Park, Ki Dong ^{[1
]}

机构：

[1] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea

[2] Yonsei Univ, Coll Med, Dept Med Engn, Brain Korea Project Med Sci 21 2, Seoul 120752, South Korea

[3] Kyung Hee Univ, Grad Sch Dent, Dept Oral Biol, Seoul, South Korea

[4] Kyung Hee Univ, Grad Sch Dent, Inst Oral Biol, Seoul, South Korea

来源：

BIOMEDICAL MATERIALS | 2008年 / 3卷 / 04期

关键词：

D O I：

10.1088/1748-6041/3/4/044104

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

An Arg-Gly-Asp (RGD) peptide-immobilized electrospun matrix of polyurethane (PU) was developed for the enhanced affinity of endothelial cells (EC). The novel PU matrix was fabricated as a vascular shape using the electrospinning technique. Then, poly(ethylene glycol) (PEG) was immobilized on the porous PU matrix as a spacer, followed by conjugating RGD peptide to the amino end group of the PEG chain. In the proliferation test of human umbilical vein endothelial cells (HUVEC) on the modified PU matrix, the RGD-immobilized porous matrix showed enhanced viability of HUVEC as compared with an unmodified surface, demonstrating that the presence of RGD peptide promoted HUVEC proliferation. In addition, the RGD-immobilized PU porous matrix revealed higher cell viability than the RGD-immobilized PU film because of the porous structure with higher surface area, indicating an advantageous property of the porous matrix for HUVEC proliferation.

引用

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