Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family

被引：208

作者：

Brown, MR ^{[1
]}

Crim, JW

Arata, RC

Cai, HN

Chun, C

Shen, P

机构：

[1] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

[2] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA

来源：

PEPTIDES | 1999年 / 20卷 / 09期

关键词：

insect; diptera; midgut; neuropeptide F; neuropeptide Y;

D O I：

10.1016/S0196-9781(99)00097-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A neuropeptide F (NPF) was isolated from the fruit fly, Drosophila melanogaster, based on a radioimmunoassay for a gut peptide from the corn earworm, Helicoverpa ten. A partial sequence was obtained from the fly peptide, and a genomic sequence coding for NPF was cloned after inverse polymerase chain reaction and shown to exist as a single genomic copy. The encoded, putative prepropeptide can be processed into an amidated NPF with 36 residues that is related to invertebrate NPF's and the neuropeptide Y family of vertebrates. In situ hybridization and immunocytochemistry showed that Drosophila NPF was expressed in the brain and midgut of By larvae and adults. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：1035 / 1042

页数：8

共 25 条

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