Absolute two-photon excitation spectra of red and far-red fluorescent probes

被引：10

作者：

Velasco, Mary Grace M. ^{[1
,2
,3
]}

Allgeyer, Edward S. ^{[2
]}

Yuan, Peng ^{[4
,5
]}

Grutzendler, Jaime ^{[4
,5
]}

Bewersdorf, Joerg ^{[1
,2
,3
]}

机构：

[1] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06511 USA

[2] Yale Univ, Dept Cell Biol, Sch Med, New Haven, CT 06511 USA

[3] Yale Univ, Integrated Grad Program Phys & Engn Biol, New Haven, CT 06511 USA

[4] Yale Univ, Dept Neurol, Sch Med, New Haven, CT 06510 USA

[5] Yale Univ, Dept Neurobiol, Sch Med, New Haven, CT 06510 USA

来源：

OPTICS LETTERS | 2015年 / 40卷 / 21期

关键词：

CROSS-SECTIONS; ABSORPTION;

D O I：

10.1364/OL.40.004915

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Efficient use of two-photon excitation (TPE) microscopy requires knowledge of the absolute TPE action cross sections (ATACSs) of fluorescent probes. However, these values are not available for recently developed dyes, which exhibit superior properties in many modern microscopy applications. We report ATACSs of five red to far-red organic dyes, ATTO 647N, STAR 635P, silicon rhodamine, ATTO 594, and ATTO 590. The dyes were found to have large ATACSs (>100 GM) at their respective wavelength peaks, thus supporting their use as bright fluorescent markers in TPE microscopy. (C) 2015 Optical Society of America

引用

页码：4915 / 4918

页数：4

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